Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KXY09_RS12650 Genome accession   NZ_CP079719
Coordinates   2607806..2608876 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain LABIM44     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2602806..2613876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KXY09_RS12600 (KXY09_12595) sipW 2602866..2603450 (-) 585 WP_371877457.1 signal peptidase I SipW -
  KXY09_RS12605 (KXY09_12600) tapA 2603422..2604093 (-) 672 WP_015240206.1 amyloid fiber anchoring/assembly protein TapA -
  KXY09_RS12610 (KXY09_12605) - 2604352..2604681 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  KXY09_RS12615 (KXY09_12610) - 2604721..2604900 (-) 180 WP_003153093.1 YqzE family protein -
  KXY09_RS12620 (KXY09_12615) comGG 2604957..2605334 (-) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  KXY09_RS12625 (KXY09_12620) comGF 2605335..2605835 (-) 501 WP_257474763.1 competence type IV pilus minor pilin ComGF -
  KXY09_RS12630 (KXY09_12625) comGE 2605744..2606058 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  KXY09_RS12635 (KXY09_12630) comGD 2606042..2606479 (-) 438 WP_052827646.1 competence type IV pilus minor pilin ComGD Machinery gene
  KXY09_RS12640 (KXY09_12635) comGC 2606469..2606777 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  KXY09_RS12645 (KXY09_12640) comGB 2606782..2607819 (-) 1038 WP_168985054.1 competence type IV pilus assembly protein ComGB Machinery gene
  KXY09_RS12650 (KXY09_12645) comGA 2607806..2608876 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  KXY09_RS12655 (KXY09_12650) - 2609073..2610023 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  KXY09_RS12660 (KXY09_12655) - 2610169..2611470 (+) 1302 WP_021494315.1 hemolysin family protein -
  KXY09_RS12665 (KXY09_12660) - 2611508..2612338 (-) 831 WP_007408317.1 STAS domain-containing protein -
  KXY09_RS12675 (KXY09_12670) - 2612545..2612925 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KXY09_RS12680 (KXY09_12675) - 2613163..2613408 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=588915 KXY09_RS12650 WP_012117985.1 2607806..2608876(-) (comGA) [Bacillus velezensis strain LABIM44]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=588915 KXY09_RS12650 WP_012117985.1 2607806..2608876(-) (comGA) [Bacillus velezensis strain LABIM44]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATTGCACTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374