Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   M1B75_RS03470 Genome accession   NZ_CP096587
Coordinates   712048..712707 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain EC6622     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 707048..717707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1B75_RS03445 (M1B75_03445) - 707704..709035 (+) 1332 WP_000778880.1 MFS transporter -
  M1B75_RS03450 (M1B75_03450) ygiN 709079..709393 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  M1B75_RS03455 (M1B75_03455) mdaB 709424..710005 (-) 582 WP_000065440.1 NADPH:quinone oxidoreductase MdaB -
  M1B75_RS03460 (M1B75_03460) ygiZ 710324..710656 (+) 333 WP_000912123.1 DUF2645 family protein -
  M1B75_RS03465 (M1B75_03465) qseC 710702..712051 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  M1B75_RS03470 (M1B75_03470) ciaR 712048..712707 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  M1B75_RS03475 (M1B75_03475) ygiW 712859..713251 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  M1B75_RS03480 (M1B75_03480) ygiV 713304..713786 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  M1B75_RS03485 (M1B75_03485) parC 714332..716590 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  M1B75_RS03490 (M1B75_03490) plsC 716823..717560 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=587159 M1B75_RS03470 WP_001221502.1 712048..712707(-) (ciaR) [Escherichia coli strain EC6622]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=587159 M1B75_RS03470 WP_001221502.1 712048..712707(-) (ciaR) [Escherichia coli strain EC6622]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365