Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilY1   Type   Machinery gene
Locus tag   EGT69_RS14715 Genome accession   NZ_CP078018
Coordinates   3068002..3071754 (-) Length   1250 a.a.
NCBI ID   WP_125297306.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ_351     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3063002..3076754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT69_RS14675 (EGT69_014675) rplS 3063178..3063549 (-) 372 WP_004660214.1 50S ribosomal protein L19 -
  EGT69_RS14680 (EGT69_014680) trmD 3063780..3064520 (-) 741 WP_004909546.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  EGT69_RS14685 (EGT69_014685) rimM 3064563..3065111 (-) 549 WP_125297304.1 ribosome maturation factor RimM -
  EGT69_RS14690 (EGT69_014690) rpsP 3065131..3065388 (-) 258 WP_004678724.1 30S ribosomal protein S16 -
  EGT69_RS14695 (EGT69_014695) - 3065672..3066044 (-) 373 Protein_2871 IS982 family transposase -
  EGT69_RS14700 (EGT69_014700) - 3066120..3066821 (+) 702 WP_004678526.1 IS1-like element ISPa14 family transposase -
  EGT69_RS14705 (EGT69_014705) - 3066981..3067436 (-) 456 WP_070153463.1 type IV pilin protein -
  EGT69_RS14710 (EGT69_014710) - 3067439..3068014 (-) 576 WP_125297305.1 type IV pilin protein -
  EGT69_RS14715 (EGT69_014715) pilY1 3068002..3071754 (-) 3753 WP_125297306.1 PilC/PilY family type IV pilus protein Machinery gene
  EGT69_RS14720 (EGT69_014720) - 3071777..3072631 (-) 855 WP_125297307.1 hypothetical protein -
  EGT69_RS14725 (EGT69_014725) pilW 3072628..3073635 (-) 1008 WP_125297308.1 PilW family protein Machinery gene
  EGT69_RS14730 (EGT69_014730) pilV 3073635..3074183 (-) 549 WP_125297309.1 type IV pilus modification protein PilV Machinery gene
  EGT69_RS14735 (EGT69_014735) - 3074183..3074683 (-) 501 WP_125297310.1 pilus assembly FimT family protein -
  EGT69_RS14740 (EGT69_014740) ispH 3074824..3075774 (-) 951 WP_125297311.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  EGT69_RS14745 (EGT69_014745) gmk 3075877..3076500 (+) 624 WP_004909574.1 guanylate kinase -

Sequence

Protein


Download         Length: 1250 a.a.        Molecular weight: 132546.71 Da        Isoelectric Point: 7.9686

>NTDB_id=586386 EGT69_RS14715 WP_125297306.1 3068002..3071754(-) (pilY1) [Acinetobacter junii strain AJ_351]
MKKFKNRLIPKNHTRHVQYGVSFVWAFTGTMLVASSIAQASDLQIYAVPTAGKKTIVMMLDTSGSMGGTDRGQTGTRLAR
LKAGMNAFLDSNNPILDEVRVGLAHYSVDGFGTAGKILVKAEKLGAVGSAQRTALKNEVSRLTASGMTPTAHAYAEAAAY
LMGTSTRSEAEIQVDVYKKEVVTTTYYRCDSLNSTNFNNGTQSCRSNGWTSLGSTPPSDLGDYTNNGSDVFYKKEMQLGP
NADSGFDKSIADSKDGLKYISPLPPAADRVSCDGQGVYVLSDGQANNSTNARSKSIMSAALDSTSFTCPSSGGLNTGGSA
SWNCMGEFAKKLFDKTTNPAGVSIQTAFVGFGNDFNNLTAPHVQQACKLSSRTQSDRTSNDSCSPGQGTYAVNAPGYGNG
GFFTTQSAQGVTDSVIAFINNLGAAPLEPLTTGAISVPLDDLNPSGLQPYGYLRALEPNPQSNKAVWTGNLKKYKVVLTG
TDAGKFAAADGTTLVYDKYGAFKTNTKDLWNSASVYQNTSYNDGGIMTLGGAYSRVPMPINGQNQDLTKNPKEYALTATP
NALRTLFTDVAATGGATLTGAANGASLLRIPESGAPTTGVAAYVLGKFSTQTTLKDFPLMVKQKLLNYLGYAVPLDEMAT
ALPTSLVTPDTPNLAMGGSIHSYPIQLTYSGTLDSTGKLTNIRSQSVLYGTMDGGLHIVDNETGEEQMVFVPAELLKNTI
ASKALVKGQDDTNAPVHGLDGAWVADPAYKAQKSSGSGNSLMKARQMNVYGGLRMSGESYYGLDVLDPKTPKLLFRVGSD
QADFSRMGQSWSKPVLTNIRYNNKITRVMIVGGGYDQCYENPKFEFGKVLSTVTNASGATVPSDFPDASCDNRTEAKGNA
VYIIDAKTGARLWWASSSTGANTSTSDMKHSIVSRISAIDRDGDGLTDHLYFGDLGGQVFRADLNNTIGTSTANFGKRVV
RLANLATTDTGAALTLGKNPRFYEAPTVTIHRQDAYSFILVGVASGNRSTPLDVYPTVGRNGMLPDSALTDRLVNNVYGV
IDRDFSKRDLISGSPTLDSIDKTLANMQKDPQQLTGNIPAVFVGATATKDGWYRSLSSKSDGTETAPGFRVAGGMKAFEE
PLAIAGNLIVPVYDPQGTGIAPQNPCLPRVVGETDRQVFGIPFGVYLKSDNTPDSSREQYTGFLTTTTDCPPGVAECNAT
PIGVGIRGVALVPKGGTGSGSVCKGLSAGPGEWDCIGQINPTRWYEKWIK

Nucleotide


Download         Length: 3753 bp        

>NTDB_id=586386 EGT69_RS14715 WP_125297306.1 3068002..3071754(-) (pilY1) [Acinetobacter junii strain AJ_351]
ATGAAAAAATTCAAAAATAGGTTAATTCCAAAGAATCACACTCGCCATGTTCAGTATGGTGTATCGTTTGTATGGGCATT
TACTGGAACGATGTTGGTTGCTTCATCCATTGCACAGGCCAGTGATTTGCAGATTTATGCAGTGCCGACCGCAGGAAAAA
AAACCATTGTTATGATGCTGGATACTTCTGGTTCGATGGGAGGGACTGATCGTGGACAAACTGGTACAAGGTTAGCCAGG
CTAAAAGCAGGTATGAATGCTTTTCTTGATAGTAATAATCCTATATTGGACGAGGTTCGGGTTGGATTGGCACATTATTC
TGTAGATGGCTTCGGTACTGCTGGTAAAATATTAGTAAAAGCAGAAAAGTTGGGGGCTGTAGGGTCCGCGCAACGTACCG
CTTTAAAAAATGAAGTAAGCCGTTTAACTGCGTCAGGGATGACACCAACAGCCCATGCCTATGCTGAAGCCGCTGCATAT
TTGATGGGAACTTCCACACGAAGTGAAGCAGAAATACAAGTGGATGTTTATAAGAAAGAAGTTGTAACCACAACTTATTA
CCGTTGCGATAGTTTAAATAGTACCAATTTTAATAATGGCACACAGTCCTGTCGTTCTAATGGTTGGACATCCTTGGGTT
CTACCCCTCCATCTGATTTAGGTGACTATACCAACAATGGTTCGGATGTATTTTATAAAAAGGAAATGCAATTAGGCCCA
AATGCTGATAGTGGTTTTGATAAATCAATTGCTGATTCTAAAGATGGTTTGAAATATATTTCACCTCTACCTCCCGCTGC
TGATCGTGTCAGTTGTGATGGGCAAGGTGTTTATGTGTTATCAGATGGTCAGGCGAATAATTCAACAAATGCGCGGTCTA
AATCCATTATGTCTGCTGCACTAGATAGCACTAGTTTTACATGCCCAAGTAGTGGAGGCCTCAATACAGGTGGGTCAGCA
TCATGGAACTGTATGGGGGAGTTCGCCAAAAAACTATTTGATAAAACAACGAATCCTGCTGGAGTTTCAATTCAAACCGC
TTTTGTGGGTTTTGGTAATGATTTTAATAACTTAACAGCACCCCATGTACAACAAGCTTGTAAATTGAGTTCACGTACTC
AAAGTGATCGTACAAGTAATGATTCTTGTTCACCAGGACAAGGAACATATGCTGTAAATGCCCCTGGTTATGGTAATGGT
GGTTTCTTTACTACTCAAAGTGCGCAAGGGGTCACCGACAGCGTAATTGCTTTTATTAACAACCTGGGAGCAGCGCCCCT
CGAGCCATTAACCACTGGGGCTATTTCTGTCCCTCTTGATGACTTAAATCCGTCAGGTTTGCAGCCTTATGGATATTTGC
GTGCTTTAGAGCCAAACCCACAAAGTAATAAGGCAGTTTGGACTGGGAACCTGAAAAAATATAAAGTGGTTTTAACAGGC
ACAGATGCAGGTAAATTCGCCGCAGCGGACGGTACAACCTTGGTCTATGATAAATATGGTGCATTTAAGACCAATACCAA
AGACCTTTGGAATAGTGCGAGTGTTTATCAAAATACCAGTTATAACGATGGTGGCATTATGACTTTGGGGGGGGCATATT
CACGAGTTCCAATGCCAATCAATGGGCAGAATCAGGATTTAACCAAGAATCCGAAAGAATATGCACTCACTGCAACGCCT
AATGCTTTGCGTACGCTATTTACGGATGTCGCTGCAACAGGTGGCGCAACATTGACAGGTGCGGCAAATGGTGCAAGTCT
GTTACGTATTCCAGAAAGTGGCGCGCCAACAACAGGTGTGGCGGCTTATGTTCTAGGTAAATTTAGTACGCAAACCACAC
TTAAAGATTTCCCCTTAATGGTGAAACAAAAATTACTGAATTATTTGGGTTATGCCGTGCCATTAGATGAAATGGCAACC
GCTTTGCCAACCTCACTAGTTACTCCAGATACCCCTAATCTGGCAATGGGAGGAAGTATTCACTCATATCCAATTCAATT
AACTTATTCAGGTACGTTAGATTCAACAGGTAAACTGACTAATATCCGTAGCCAATCTGTTTTGTATGGAACGATGGATG
GTGGTTTGCATATTGTGGACAATGAAACGGGTGAGGAACAAATGGTCTTCGTTCCTGCTGAATTGCTCAAAAATACCATT
GCTTCCAAAGCTTTGGTTAAAGGGCAGGATGATACGAATGCACCTGTACATGGTTTGGATGGAGCATGGGTGGCTGATCC
TGCCTATAAAGCCCAAAAGTCGAGTGGGTCAGGTAACAGTTTGATGAAAGCACGTCAAATGAATGTCTATGGTGGCTTAC
GCATGAGCGGTGAAAGCTATTATGGTTTGGACGTATTAGATCCGAAAACGCCGAAGTTGTTATTTAGAGTGGGCTCAGAT
CAAGCTGATTTTAGCCGCATGGGGCAATCATGGTCTAAGCCTGTATTGACCAATATTCGTTATAACAACAAAATTACCCG
TGTGATGATTGTGGGTGGAGGCTATGATCAATGTTATGAAAATCCTAAGTTTGAATTTGGTAAAGTATTGTCTACTGTGA
CCAATGCAAGTGGCGCGACAGTACCAAGTGACTTCCCTGATGCTTCTTGTGATAATCGCACTGAGGCAAAAGGAAATGCT
GTTTATATTATTGATGCAAAAACAGGTGCGCGTTTATGGTGGGCGAGTAGTTCTACTGGTGCGAATACCTCTACTAGTGA
TATGAAGCATAGTATTGTGAGTCGTATCAGTGCCATTGACCGTGATGGTGATGGTTTGACAGATCATCTTTATTTTGGTG
ATTTAGGTGGCCAAGTCTTCCGTGCAGATCTGAATAATACAATCGGAACTTCAACTGCAAACTTTGGTAAGCGCGTGGTA
CGTTTGGCAAATTTAGCAACAACTGATACAGGGGCTGCTTTAACACTTGGAAAAAATCCACGTTTCTATGAAGCGCCAAC
GGTCACCATTCATCGTCAGGATGCATATAGTTTTATATTGGTAGGCGTGGCATCAGGAAACCGTAGTACGCCATTGGACG
TTTATCCAACTGTTGGACGTAATGGGATGCTGCCAGATAGTGCACTTACAGATCGCTTGGTGAATAATGTGTATGGTGTC
ATTGACCGTGACTTTTCCAAGAGAGATCTGATTAGTGGTAGCCCAACACTGGATTCTATAGATAAAACTTTAGCGAATAT
GCAGAAAGATCCACAACAACTTACAGGTAATATTCCTGCGGTGTTTGTTGGGGCAACGGCAACCAAAGATGGATGGTATC
GTTCATTATCAAGTAAGAGTGATGGTACAGAAACAGCGCCAGGATTCCGTGTCGCAGGGGGGATGAAAGCCTTTGAAGAG
CCTCTTGCAATTGCAGGTAATTTAATCGTGCCTGTATATGATCCGCAGGGTACAGGCATTGCACCACAGAATCCATGCTT
ACCACGAGTGGTAGGCGAGACAGATCGTCAGGTATTTGGTATTCCATTTGGTGTGTATTTGAAGTCAGACAATACCCCTG
ATAGCAGTAGAGAGCAATATACAGGTTTCTTGACAACAACAACCGATTGCCCACCAGGAGTTGCAGAATGTAATGCTACA
CCAATTGGTGTAGGGATTCGCGGTGTTGCTTTAGTTCCAAAAGGAGGAACAGGAAGTGGAAGTGTCTGTAAAGGATTGTC
TGCTGGTCCTGGTGAGTGGGATTGTATAGGACAGATTAACCCAACACGGTGGTATGAAAAATGGATCAAATAA

Domains

Predicted by InterproScan.

von Willebrand factor type A domain (VWA_2)

(56-165)

Neisseria PilC beta-propeller domain (T4P_PilY1)

(667-946)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilY1 Acinetobacter baumannii D1279779

55.753

100

0.578