Detailed information NTG0586083

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MZN68_RS09760
• Genome accession: NZ_CP096117
• Coordinates: 1936657..1937883 (-)
• Length: 408 a.a.
• NCBI ID: WP_002218483.1
• Uniprot ID: Q9K1N4
• Organism: Neisseria meningitidis strain DFS-1910310016
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1931657..1942883

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MZN68_RS09750 (MZN68_09580)	-	1932865..1933821 (+)	957	WP_002212521.1	IS110-like element ISNgo3 family transposase	-
MZN68_RS09755 (MZN68_09585)	yccS	1934221..1936371 (-)	2151	WP_002220096.1	YccS family putative transporter	-
MZN68_RS09760 (MZN68_09590)	pilU	1936657..1937883 (-)	1227	WP_002218483.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MZN68_RS09765 (MZN68_09595)	pilT	1938046..1939089 (-)	1044	WP_002226887.1	type IV pilus twitching motility protein PilT	Machinery gene
MZN68_RS09770 (MZN68_09600)	-	1939209..1939895 (+)	687	WP_002242149.1	YggS family pyridoxal phosphate-dependent enzyme	-
MZN68_RS09775 (MZN68_09605)	-	1939895..1940302 (+)	408	WP_002242150.1	hypothetical protein	-
MZN68_RS09780 (MZN68_09610)	proC	1940351..1941142 (+)	792	WP_002215263.1	pyrroline-5-carboxylate reductase	-
MZN68_RS09785 (MZN68_09615)	dksA	1941289..1941705 (+)	417	WP_002215267.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45674.28 Da
• Isoelectric Point: 6.9640

>NTDB_id=586083 MZN68_RS09760 WP_002218483.1 1936657..1937883(-) (pilU) [Neisseria meningitidis strain DFS-1910310016]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=586083 MZN68_RS09760 WP_002218483.1 1936657..1937883(-) (pilU) [Neisseria meningitidis strain DFS-1910310016]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACAGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q9K1N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.598	88.48	0.395
pilU	Pseudomonas stutzeri DSM 10701	44.663	87.255	0.39
pilU	Vibrio cholerae strain A1552	46.429	82.353	0.382