Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   KU511_RS03535 Genome accession   NZ_CP077928
Coordinates   665748..666245 (+) Length   165 a.a.
NCBI ID   WP_001796472.1    Uniprot ID   A0A5F0HJ55
Organism   Staphylococcus aureus strain 196     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 660748..671245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU511_RS03495 (KU511_03490) - 660792..661778 (+) 987 WP_000161307.1 ROK family glucokinase -
  KU511_RS03500 (KU511_03495) - 661778..662107 (+) 330 WP_001018870.1 MTH1187 family thiamine-binding protein -
  KU511_RS03505 (KU511_03500) - 662104..662727 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  KU511_RS03510 (KU511_03505) comGA 662779..663753 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU511_RS03515 (KU511_03510) comGB 663725..664795 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU511_RS03520 (KU511_03515) comGC 664809..665120 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  KU511_RS03525 (KU511_03520) comGD 665098..665544 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU511_RS03530 (KU511_03525) comGE 665531..665830 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU511_RS03535 (KU511_03530) comGF 665748..666245 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU511_RS03540 (KU511_03535) - 666342..666488 (+) 147 WP_001789879.1 hypothetical protein -
  KU511_RS03545 (KU511_03540) - 666478..667002 (+) 525 WP_001015120.1 shikimate kinase -
  KU511_RS03550 (KU511_03545) gcvT 667161..668252 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  KU511_RS03555 (KU511_03550) gcvPA 668272..669618 (+) 1347 WP_031928187.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  KU511_RS03560 (KU511_03555) gcvPB 669611..671083 (+) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19169.89 Da        Isoelectric Point: 10.1358

>NTDB_id=585418 KU511_RS03535 WP_001796472.1 665748..666245(+) (comGF) [Staphylococcus aureus strain 196]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=585418 KU511_RS03535 WP_001796472.1 665748..666245(+) (comGF) [Staphylococcus aureus strain 196]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

95.758

100

0.958