Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KU511_RS03510 Genome accession   NZ_CP077928
Coordinates   662779..663753 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain 196     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 657779..668753
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KU511_RS03475 (KU511_03470) rpmG 658239..658388 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  KU511_RS03480 (KU511_03475) - 658597..659136 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  KU511_RS03485 (KU511_03480) - 659148..660611 (+) 1464 WP_001017870.1 rhomboid family protein -
  KU511_RS03490 (KU511_03485) - 660592..660795 (+) 204 WP_000087561.1 YqgQ family protein -
  KU511_RS03495 (KU511_03490) - 660792..661778 (+) 987 WP_000161307.1 ROK family glucokinase -
  KU511_RS03500 (KU511_03495) - 661778..662107 (+) 330 WP_001018870.1 MTH1187 family thiamine-binding protein -
  KU511_RS03505 (KU511_03500) - 662104..662727 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  KU511_RS03510 (KU511_03505) comGA 662779..663753 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  KU511_RS03515 (KU511_03510) comGB 663725..664795 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  KU511_RS03520 (KU511_03515) comGC 664809..665120 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  KU511_RS03525 (KU511_03520) comGD 665098..665544 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  KU511_RS03530 (KU511_03525) comGE 665531..665830 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KU511_RS03535 (KU511_03530) comGF 665748..666245 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  KU511_RS03540 (KU511_03535) - 666342..666488 (+) 147 WP_001789879.1 hypothetical protein -
  KU511_RS03545 (KU511_03540) - 666478..667002 (+) 525 WP_001015120.1 shikimate kinase -
  KU511_RS03550 (KU511_03545) gcvT 667161..668252 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=585413 KU511_RS03510 WP_000697220.1 662779..663753(+) (comGA) [Staphylococcus aureus strain 196]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=585413 KU511_RS03510 WP_000697220.1 662779..663753(+) (comGA) [Staphylococcus aureus strain 196]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGACTTGATGTATCTACACAACAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTGATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1