Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MW400_RS21440 Genome accession   NZ_CP095533
Coordinates   4573658..4574317 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain GN02461     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4568658..4579317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW400_RS21425 (MW400_21415) ygiS 4570863..4572470 (-) 1608 WP_001327858.1 ABC transporter substrate-binding protein -
  MW400_RS21430 (MW400_21420) ygiV 4572579..4573061 (-) 483 WP_000183494.1 AraC family transcriptional regulator -
  MW400_RS21435 (MW400_21425) ygiW 4573114..4573506 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  MW400_RS21440 (MW400_21430) ciaR 4573658..4574317 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  MW400_RS21445 (MW400_21435) qseC 4574314..4575663 (+) 1350 WP_000673373.1 quorum sensing histidine kinase QseC -
  MW400_RS21450 (MW400_21440) ygiZ 4575712..4576041 (-) 330 WP_000914694.1 DUF2645 family protein -
  MW400_RS21455 (MW400_21445) - 4576048..4576758 (-) 711 WP_000834027.1 hypothetical protein -
  MW400_RS21460 (MW400_21450) - 4576761..4577240 (-) 480 WP_000065334.1 Hcp family type VI secretion system effector -
  MW400_RS21465 (MW400_21455) mdaB 4577490..4578071 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  MW400_RS21470 (MW400_21460) ygiN 4578102..4578416 (+) 315 WP_000958604.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=583874 MW400_RS21440 WP_001221493.1 4573658..4574317(+) (ciaR) [Escherichia coli strain GN02461]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=583874 MW400_RS21440 WP_001221493.1 4573658..4574317(+) (ciaR) [Escherichia coli strain GN02461]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365