Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MW397_RS19330 Genome accession   NZ_CP095529
Coordinates   4068870..4069529 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain GN06156     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4063870..4074529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MW397_RS19310 (MW397_19300) plsC 4064016..4064753 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  MW397_RS19315 (MW397_19305) parC 4064987..4067245 (-) 2259 WP_001281919.1 DNA topoisomerase IV subunit A -
  MW397_RS19320 (MW397_19310) ygiV 4067791..4068273 (-) 483 WP_000183504.1 AraC family transcriptional regulator -
  MW397_RS19325 (MW397_19315) ygiW 4068326..4068718 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  MW397_RS19330 (MW397_19320) ciaR 4068870..4069529 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  MW397_RS19335 (MW397_19325) qseC 4069526..4070872 (+) 1347 WP_029305530.1 quorum sensing histidine kinase QseC -
  MW397_RS19340 (MW397_19330) mdaB 4070985..4071566 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  MW397_RS19345 (MW397_19335) ygiN 4071597..4071911 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  MW397_RS19350 (MW397_19340) - 4071956..4072843 (-) 888 WP_000614953.1 MurR/RpiR family transcriptional regulator -
  MW397_RS19355 (MW397_19345) - 4072840..4073787 (-) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=583783 MW397_RS19330 WP_001221493.1 4068870..4069529(+) (ciaR) [Escherichia coli strain GN06156]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=583783 MW397_RS19330 WP_001221493.1 4068870..4069529(+) (ciaR) [Escherichia coli strain GN06156]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACTCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365