Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MU524_RS03510 Genome accession   NZ_CP095271
Coordinates   717395..718054 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain EC9682     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 712395..723054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU524_RS03480 (MU524_03480) ygiN 713296..713610 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  MU524_RS03485 (MU524_03485) mdaB 713641..714222 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  MU524_RS03490 (MU524_03490) - 714472..714951 (+) 480 WP_000069056.1 Hcp family type VI secretion system effector -
  MU524_RS03495 (MU524_03495) - 714954..715664 (+) 711 WP_000834021.1 hypothetical protein -
  MU524_RS03500 (MU524_03500) ygiZ 715671..716003 (+) 333 WP_000914696.1 DUF2645 family protein -
  MU524_RS03505 (MU524_03505) qseC 716049..717398 (-) 1350 WP_000673382.1 quorum sensing histidine kinase QseC -
  MU524_RS03510 (MU524_03510) ciaR 717395..718054 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  MU524_RS03515 (MU524_03515) ygiW 718206..718598 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  MU524_RS03520 (MU524_03520) ygiV 718651..719133 (+) 483 WP_000183493.1 GyrI-like domain-containing protein -
  MU524_RS03525 (MU524_03525) ygiS 719242..720849 (+) 1608 WP_001350728.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=582818 MU524_RS03510 WP_001221493.1 717395..718054(-) (ciaR) [Escherichia coli strain EC9682]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=582818 MU524_RS03510 WP_001221493.1 717395..718054(-) (ciaR) [Escherichia coli strain EC9682]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365