Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VB13_RS00680 Genome accession   NZ_CP077685
Coordinates   104890..105324 (+) Length   144 a.a.
NCBI ID   WP_009880359.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain M49 591     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99890..110324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VB13_RS00650 (VB13_00680) - 101559..101924 (+) 366 WP_002986560.1 DUF1033 family protein -
  VB13_RS00655 (VB13_00685) comYA 102017..102955 (+) 939 WP_009880361.1 competence type IV pilus ATPase ComGA Machinery gene
  VB13_RS00660 (VB13_00690) comYB 102891..103925 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  VB13_RS00665 (VB13_00695) comGC 103927..104253 (+) 327 WP_012560386.1 competence type IV pilus major pilin ComGC Machinery gene
  VB13_RS00670 (VB13_00700) comGD 104228..104656 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  VB13_RS00675 (VB13_00705) comGE 104613..104897 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  VB13_RS00680 (VB13_00710) comYF 104890..105324 (+) 435 WP_009880359.1 competence type IV pilus minor pilin ComGF Machinery gene
  VB13_RS00685 (VB13_00715) comGG 105308..105634 (+) 327 WP_009880358.1 competence type IV pilus minor pilin ComGG -
  VB13_RS00690 (VB13_00720) comYH 105732..106685 (+) 954 WP_009880357.1 class I SAM-dependent methyltransferase Machinery gene
  VB13_RS00695 (VB13_00725) - 106744..107940 (+) 1197 WP_009880356.1 acetate kinase -
  VB13_RS00700 - 108126..108434 (+) 309 Protein_88 hypothetical protein -
  VB13_RS00705 (VB13_00730) proC 108517..109287 (-) 771 WP_009880354.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16657.29 Da        Isoelectric Point: 10.0822

>NTDB_id=582216 VB13_RS00680 WP_009880359.1 104890..105324(+) (comYF) [Streptococcus pyogenes strain M49 591]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSEARFCKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=582216 VB13_RS00680 WP_009880359.1 104890..105324(+) (comYF) [Streptococcus pyogenes strain M49 591]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTACCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGAAGCAAGATTTTGCAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGATATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.36

96.528

0.486
  comYF Streptococcus mutans UA159

49.64

96.528

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396