Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KTT68_RS12160 Genome accession   NZ_CP077672
Coordinates   2522854..2523468 (-) Length   204 a.a.
NCBI ID   WP_045208286.1    Uniprot ID   -
Organism   Bacillus velezensis strain SWUJ1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2517854..2528468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KTT68_RS12130 rpsT 2518157..2518423 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  KTT68_RS12135 holA 2518440..2519481 (-) 1042 Protein_2344 DNA polymerase III subunit delta -
  KTT68_RS12140 - 2519521..2519672 (-) 152 Protein_2345 hypothetical protein -
  KTT68_RS12145 - 2519713..2519847 (+) 135 WP_003152870.1 YqzM family protein -
  KTT68_RS12150 comEC 2519866..2522217 (-) 2352 WP_045208284.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KTT68_RS12155 - 2522218..2522787 (-) 570 WP_003152868.1 ComE operon protein 2 -
  KTT68_RS12160 comEA 2522854..2523468 (-) 615 WP_045208286.1 helix-hairpin-helix domain-containing protein Machinery gene
  KTT68_RS12165 comER 2523527..2524348 (+) 822 WP_012118027.1 late competence protein ComER -
  KTT68_RS12170 - 2524417..2525154 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  KTT68_RS12175 rsfS 2525151..2525507 (-) 357 WP_007408260.1 ribosome silencing factor -
  KTT68_RS12180 yqeK 2525525..2526085 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KTT68_RS12185 - 2526075..2526644 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  KTT68_RS12190 yhbY 2526655..2526945 (-) 291 WP_045208288.1 ribosome assembly RNA-binding protein YhbY -
  KTT68_RS12195 aroE 2526939..2527781 (-) 843 WP_032873946.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22323.60 Da        Isoelectric Point: 7.7754

>NTDB_id=582087 KTT68_RS12160 WP_045208286.1 2522854..2523468(-) (comEA) [Bacillus velezensis strain SWUJ1]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTTDDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQVISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=582087 KTT68_RS12160 WP_045208286.1 2522854..2523468(-) (comEA) [Bacillus velezensis strain SWUJ1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCACAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGTCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGTGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA Lactococcus lactis subsp. cremoris KW2

33.784

100

0.368
  comEA Latilactobacillus sakei subsp. sakei 23K

31.897

100

0.363
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363