Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KTT68_RS11735 Genome accession   NZ_CP077672
Coordinates   2446339..2447409 (-) Length   356 a.a.
NCBI ID   WP_012117985.1    Uniprot ID   A0A411A7V3
Organism   Bacillus velezensis strain SWUJ1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2441339..2452409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KTT68_RS11685 sipW 2441399..2441983 (-) 585 WP_007408328.1 signal peptidase I SipW -
  KTT68_RS11690 tapA 2441955..2442626 (-) 672 WP_020954230.1 amyloid fiber anchoring/assembly protein TapA -
  KTT68_RS11695 - 2442885..2443214 (+) 330 WP_020954231.1 DUF3889 domain-containing protein -
  KTT68_RS11700 - 2443254..2443433 (-) 180 WP_003153093.1 YqzE family protein -
  KTT68_RS11705 comGG 2443490..2443867 (-) 378 WP_039063315.1 competence type IV pilus minor pilin ComGG -
  KTT68_RS11710 comGF 2443868..2444368 (-) 501 WP_258038902.1 competence type IV pilus minor pilin ComGF -
  KTT68_RS11715 comGE 2444277..2444591 (-) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  KTT68_RS11720 comGD 2444575..2445012 (-) 438 WP_007408322.1 competence type IV pilus minor pilin ComGD Machinery gene
  KTT68_RS11725 comGC 2445002..2445310 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  KTT68_RS11730 comGB 2445315..2446352 (-) 1038 WP_032866436.1 competence type IV pilus assembly protein ComGB Machinery gene
  KTT68_RS11735 comGA 2446339..2447409 (-) 1071 WP_012117985.1 competence type IV pilus ATPase ComGA Machinery gene
  KTT68_RS11740 - 2447601..2448551 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  KTT68_RS11745 - 2448697..2449998 (+) 1302 WP_039063318.1 hemolysin family protein -
  KTT68_RS11750 - 2450036..2450866 (-) 831 WP_015240214.1 STAS domain-containing protein -
  KTT68_RS11760 - 2451073..2451453 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KTT68_RS11765 - 2451690..2451935 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.86 Da        Isoelectric Point: 9.4784

>NTDB_id=582083 KTT68_RS11735 WP_012117985.1 2446339..2447409(-) (comGA) [Bacillus velezensis strain SWUJ1]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=582083 KTT68_RS11735 WP_012117985.1 2446339..2447409(-) (comGA) [Bacillus velezensis strain SWUJ1]
GTGAAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCCTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A7V3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374