Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KP952_RS13015 Genome accession   NZ_CP076731
Coordinates   2443197..2443814 (-) Length   205 a.a.
NCBI ID   WP_046160608.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis strain Miz-8     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2438197..2448814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KP952_RS12985 (KP952_12985) rpsT 2438500..2438766 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  KP952_RS12990 (KP952_12990) holA 2438781..2439824 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  KP952_RS12995 (KP952_12995) - 2439864..2440013 (-) 150 WP_003229985.1 hypothetical protein -
  KP952_RS13000 (KP952_13000) yqzM 2440054..2440188 (+) 135 WP_003229983.1 YqzM family protein -
  KP952_RS13005 (KP952_13005) comEC 2440228..2442557 (-) 2330 Protein_2536 DNA internalization-related competence protein ComEC/Rec2 -
  KP952_RS13010 (KP952_13010) comEB 2442561..2443130 (-) 570 WP_003229978.1 ComE operon protein 2 -
  KP952_RS13015 (KP952_13015) comEA 2443197..2443814 (-) 618 WP_046160608.1 competence protein ComEA Machinery gene
  KP952_RS13020 (KP952_13020) comER 2443898..2444719 (+) 822 WP_032726195.1 late competence protein ComER -
  KP952_RS13025 (KP952_13025) yqeM 2444785..2445528 (-) 744 WP_069837641.1 class I SAM-dependent DNA methyltransferase -
  KP952_RS13030 (KP952_13030) rsfS 2445525..2445881 (-) 357 WP_014480315.1 ribosome silencing factor -
  KP952_RS13035 (KP952_13035) yqeK 2445899..2446459 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KP952_RS13040 (KP952_13040) nadD 2446449..2447018 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  KP952_RS13045 (KP952_13045) yhbY 2447030..2447320 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  KP952_RS13050 (KP952_13050) aroE 2447314..2448156 (-) 843 WP_014480317.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21738.48 Da        Isoelectric Point: 4.7284

>NTDB_id=577771 KP952_RS13015 WP_046160608.1 2443197..2443814(-) (comEA) [Bacillus subtilis subsp. subtilis strain Miz-8]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=577771 KP952_RS13015 WP_046160608.1 2443197..2443814(-) (comEA) [Bacillus subtilis subsp. subtilis strain Miz-8]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

97.561

100

0.976
  comEA Staphylococcus aureus MW2

35.909

100

0.385
  comEA Staphylococcus aureus N315

35

100

0.376