Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D650_RS08805 Genome accession   NC_020833
Coordinates   1717569..1718048 (-) Length   159 a.a.
NCBI ID   WP_006248401.1    Uniprot ID   A0A249A0S4
Organism   Mannheimia haemolytica USDA-ARS-USMARC-183     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1712569..1723048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D650_RS08770 (D650_17710) lptE 1712849..1713337 (+) 489 WP_006248408.1 LPS assembly lipoprotein LptE -
  D650_RS08775 (D650_17720) holA 1713380..1714405 (+) 1026 WP_006253426.1 DNA polymerase III subunit delta -
  D650_RS08780 (D650_17730) yacG 1714406..1714591 (-) 186 WP_006248406.1 DNA gyrase inhibitor YacG -
  D650_RS08785 (D650_17740) coaE 1714573..1715208 (-) 636 WP_006248405.1 dephospho-CoA kinase -
  D650_RS08790 (D650_17750) - 1715244..1715900 (-) 657 WP_006248404.1 prepilin peptidase -
  D650_RS08795 (D650_17760) pilC 1715903..1717099 (-) 1197 WP_006248403.1 type II secretion system F family protein Machinery gene
  D650_RS08800 (D650_17770) - 1717105..1717548 (-) 444 Protein_1706 pilus assembly protein PilB -
  D650_RS08805 (D650_17780) pilA 1717569..1718048 (-) 480 WP_006248401.1 prepilin peptidase-dependent pilin Machinery gene
  D650_RS08810 (D650_17790) radA 1718229..1719608 (+) 1380 WP_006248399.1 DNA repair protein RadA -
  D650_RS08815 (D650_17800) - 1719663..1719893 (-) 231 WP_006248398.1 YgjV family protein -
  D650_RS08820 (D650_17810) folA 1720013..1720507 (-) 495 WP_006248397.1 type 3 dihydrofolate reductase -
  D650_RS08825 (D650_17830) proB 1720619..1721703 (+) 1085 Protein_1711 glutamate 5-kinase -
  D650_RS08830 (D650_17840) - 1721703..1722467 (+) 765 WP_006248395.1 TSUP family transporter -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 16841.15 Da        Isoelectric Point: 8.7964

>NTDB_id=57576 D650_RS08805 WP_006248401.1 1717569..1718048(-) (pilA) [Mannheimia haemolytica USDA-ARS-USMARC-183]
MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNSYTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSG
GTNGIQANKTSSNTTKYLNSIAVTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG

Nucleotide


Download         Length: 480 bp        

>NTDB_id=57576 D650_RS08805 WP_006248401.1 1717569..1718048(-) (pilA) [Mannheimia haemolytica USDA-ARS-USMARC-183]
ATGCAAAAAATTGTACATTTTAGACCGCTTGGCATTCGCCAACTGCATCGGGCATTTACCCTCATTGAGTTGATGATCGT
GATTGCGATTATTGCCATTCTTGCCACTATTGCGATCCCTTCCTATAACTCATACACCCAAAAGGCGGCGTTATCGGAGT
TATTACAAGCATCTGCTTCGTATAAATCAGACGTTGAAGTCTGTATTTACAACAGTGGCGGGTTGGATAACTGCTCCGGT
GGCACGAATGGGATTCAGGCGAATAAAACCAGTAGTAATACCACTAAATACCTAAACTCAATTGCGGTAACAAAAGGCGT
GATTACGGTAACCGGCAAAGGCTCTCTGAATGGTTATGGCTATACCTTAACACCGTCGTTCTCAAATAATACGATTAGTT
GGACGGCAAGTTGTAGTGGCAATGATACGAGCCTATTTCCAGCTAATTTCTGTAGCACAACTGTTACACCAAACGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(48-148)

Prokaryotic N-terminal methylation motif (N_methyl)

(16-38)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A249A0S4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

66.038

100

0.66
  pilA Haemophilus influenzae 86-028NP

56.429

88.05

0.497
  pilA Haemophilus influenzae Rd KW20

54.93

89.308

0.491

Multiple sequence alignment