Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KOM03_RS17000 Genome accession   NZ_CP076450
Coordinates   3493332..3494402 (-) Length   356 a.a.
NCBI ID   WP_007408320.1    Uniprot ID   -
Organism   Bacillus velezensis strain GMEKP1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3488332..3499402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOM03_RS16950 (KOM03_16950) sipW 3488392..3488976 (-) 585 WP_060562614.1 signal peptidase I SipW -
  KOM03_RS16955 (KOM03_16955) tapA 3488948..3489619 (-) 672 WP_060562615.1 amyloid fiber anchoring/assembly protein TapA -
  KOM03_RS16960 (KOM03_16960) - 3489878..3490207 (+) 330 WP_060562616.1 DUF3889 domain-containing protein -
  KOM03_RS16965 (KOM03_16965) - 3490247..3490426 (-) 180 WP_003153093.1 YqzE family protein -
  KOM03_RS16970 (KOM03_16970) comGG 3490483..3490860 (-) 378 WP_060562617.1 competence type IV pilus minor pilin ComGG Machinery gene
  KOM03_RS16975 (KOM03_16975) comGF 3490861..3491361 (-) 501 WP_014305411.1 competence type IV pilus minor pilin ComGF -
  KOM03_RS16980 (KOM03_16980) comGE 3491270..3491584 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  KOM03_RS16985 (KOM03_16985) comGD 3491568..3492005 (-) 438 WP_012117983.1 competence type IV pilus minor pilin ComGD Machinery gene
  KOM03_RS16990 (KOM03_16990) comGC 3491995..3492303 (-) 309 WP_079891096.1 competence type IV pilus major pilin ComGC Machinery gene
  KOM03_RS16995 (KOM03_16995) comGB 3492308..3493345 (-) 1038 WP_060562618.1 competence type IV pilus assembly protein ComGB Machinery gene
  KOM03_RS17000 (KOM03_17000) comGA 3493332..3494402 (-) 1071 WP_007408320.1 competence type IV pilus ATPase ComGA Machinery gene
  KOM03_RS17005 (KOM03_17005) - 3494595..3495545 (-) 951 WP_007408319.1 magnesium transporter CorA family protein -
  KOM03_RS17010 (KOM03_17010) - 3495691..3496992 (+) 1302 WP_060562619.1 hemolysin family protein -
  KOM03_RS17015 (KOM03_17015) - 3497030..3497860 (-) 831 WP_015240214.1 STAS domain-containing protein -
  KOM03_RS17025 (KOM03_17025) - 3498067..3498447 (-) 381 WP_060562620.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KOM03_RS17030 (KOM03_17030) - 3498685..3498930 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40530.79 Da        Isoelectric Point: 9.1996

>NTDB_id=575497 KOM03_RS17000 WP_007408320.1 3493332..3494402(-) (comGA) [Bacillus velezensis strain GMEKP1]
MENIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=575497 KOM03_RS17000 WP_007408320.1 3493332..3494402(-) (comGA) [Bacillus velezensis strain GMEKP1]
GTGGAAAATATAGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTAATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGTCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTTCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGTTTTCAGGAAGCAAAGGGAGAG
CACGCGAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374