Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KNN15_RS01925 Genome accession   NZ_CP076325
Coordinates   361796..362422 (+) Length   208 a.a.
NCBI ID   WP_273107705.1    Uniprot ID   -
Organism   MAG: Thermus antranikianii isolate RBS10-92     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356796..367422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KNN15_RS01900 (KNN15_01875) - 356831..358246 (+) 1416 WP_273105940.1 HD-GYP domain-containing protein -
  KNN15_RS01905 (KNN15_01880) - 358282..358791 (+) 510 WP_273107703.1 DUF5317 domain-containing protein -
  KNN15_RS01910 (KNN15_01885) - 358861..359865 (+) 1005 WP_273105943.1 homoisocitrate dehydrogenase -
  KNN15_RS01915 (KNN15_01890) pilM 360057..361190 (+) 1134 WP_273105946.1 type IV pilus assembly protein PilM Machinery gene
  KNN15_RS01920 (KNN15_01895) pilN 361183..361806 (+) 624 WP_028493409.1 competence protein Machinery gene
  KNN15_RS01925 (KNN15_01900) pilO 361796..362422 (+) 627 WP_273107705.1 type 4a pilus biogenesis protein PilO Machinery gene
  KNN15_RS01930 (KNN15_01905) pilW 362419..363390 (+) 972 WP_273105950.1 competence protein Machinery gene
  KNN15_RS01935 (KNN15_01910) pilQ 363387..365666 (+) 2280 WP_273105953.1 secretin N-terminal domain-containing protein Machinery gene
  KNN15_RS01940 - 365755..366270 (-) 516 WP_273105956.1 DDE transposase family protein -
  KNN15_RS01945 - 366291..366845 (-) 555 WP_014629763.1 transposase family protein -

Sequence

Protein


Download         Length: 208 a.a.        Molecular weight: 22999.44 Da        Isoelectric Point: 9.4989

>NTDB_id=574669 KNN15_RS01925 WP_273107705.1 361796..362422(+) (pilO) [MAG: Thermus antranikianii isolate RBS10-92]
MLARLGQREWALIAIALTLVVALLWYFFLIVPMRQETESVRQEINALIPERDKGRQAQRALPELRATIAELQAERQAFLR
ALPKEERLSQVLNEILTEAIRSGVTVRSFTRSPTSAPVPEVRAVNLALSLEAPFPETYAYLKRLEGLSRFSSLSGLNLSV
QGQDLNPLLSTSLTLTLYMLAKDLSQSEGNPQTQGTPPSQTTGQGGGR

Nucleotide


Download         Length: 627 bp        

>NTDB_id=574669 KNN15_RS01925 WP_273107705.1 361796..362422(+) (pilO) [MAG: Thermus antranikianii isolate RBS10-92]
GTGCTCGCTAGACTGGGACAGCGGGAGTGGGCCCTGATCGCCATCGCCCTCACCTTGGTGGTGGCCCTCCTCTGGTACTT
TTTCCTCATCGTCCCCATGCGTCAGGAAACGGAAAGCGTGCGCCAGGAGATTAACGCCCTTATCCCCGAGCGGGATAAAG
GAAGGCAAGCCCAGCGCGCCCTTCCAGAACTCCGGGCCACCATCGCTGAGCTGCAAGCCGAGCGTCAGGCCTTTCTGAGG
GCGCTTCCCAAGGAGGAACGGCTTTCCCAGGTTCTGAACGAGATCCTGACCGAGGCCATAAGGAGCGGGGTCACGGTGCG
CTCCTTCACCCGCTCCCCCACCTCGGCTCCAGTACCGGAGGTGCGGGCGGTGAACCTGGCCCTTTCCCTCGAGGCGCCCT
TCCCCGAAACCTACGCCTATCTGAAGCGCCTGGAAGGGCTTTCCCGCTTCAGCTCCCTTTCCGGGCTCAACCTCAGCGTC
CAGGGTCAGGACTTAAATCCCCTCCTCTCCACCAGCTTGACGCTGACCCTCTACATGCTGGCCAAAGATCTTAGCCAGTC
CGAAGGAAATCCCCAGACGCAAGGAACTCCTCCCTCGCAGACAACAGGTCAAGGAGGTGGTCGGTGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(46-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

71.579

91.346

0.654