Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KM776_RS13560 Genome accession   NZ_CP076290
Coordinates   2753935..2754549 (-) Length   204 a.a.
NCBI ID   WP_069013538.1    Uniprot ID   -
Organism   Bacillus velezensis strain Q12     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2748935..2759549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KM776_RS13530 (KM776_13530) rpsT 2749235..2749501 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  KM776_RS13535 (KM776_13535) holA 2749518..2750559 (-) 1042 Protein_2649 DNA polymerase III subunit delta -
  KM776_RS13540 (KM776_13540) - 2750599..2750753 (-) 155 Protein_2650 hypothetical protein -
  KM776_RS13545 (KM776_13545) - 2750794..2750928 (+) 135 WP_003152870.1 YqzM family protein -
  KM776_RS13550 (KM776_13550) comEC 2750947..2753298 (-) 2352 WP_069013539.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KM776_RS13555 (KM776_13555) - 2753299..2753868 (-) 570 WP_003152868.1 ComE operon protein 2 -
  KM776_RS13560 (KM776_13560) comEA 2753935..2754549 (-) 615 WP_069013538.1 helix-hairpin-helix domain-containing protein Machinery gene
  KM776_RS13565 (KM776_13565) comER 2754608..2755429 (+) 822 WP_014305449.1 late competence protein ComER -
  KM776_RS13570 (KM776_13570) - 2755498..2756235 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  KM776_RS13575 (KM776_13575) rsfS 2756232..2756588 (-) 357 WP_003152864.1 ribosome silencing factor -
  KM776_RS13580 (KM776_13580) yqeK 2756585..2757166 (-) 582 WP_057080474.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KM776_RS13585 (KM776_13585) - 2757156..2757725 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  KM776_RS13590 (KM776_13590) yhbY 2757736..2758026 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  KM776_RS13595 (KM776_13595) aroE 2758020..2758862 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22164.30 Da        Isoelectric Point: 6.0433

>NTDB_id=574479 KM776_RS13560 WP_069013538.1 2753935..2754549(-) (comEA) [Bacillus velezensis strain Q12]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=574479 KM776_RS13560 WP_069013538.1 2753935..2754549(-) (comEA) [Bacillus velezensis strain Q12]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363