Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   G8O86_RS00290 Genome accession   NZ_CP075877
Coordinates   64763..65245 (+) Length   160 a.a.
NCBI ID   WP_003722552.1    Uniprot ID   A0A3T2EU60
Organism   Listeria monocytogenes strain 2HF15     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 64763..91472 64763..65245 within 0


Gene organization within MGE regions


Location: 64763..91472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8O86_RS00290 ssbA 64763..65245 (+) 483 WP_003722552.1 single-stranded DNA-binding protein Machinery gene
  G8O86_RS00295 - 65264..65446 (+) 183 WP_009924219.1 hypothetical protein -
  G8O86_RS00300 - 65391..65795 (+) 405 WP_010989958.1 DUF1064 domain-containing protein -
  G8O86_RS00305 - 65799..66182 (+) 384 WP_009924217.1 DUF2481 family protein -
  G8O86_RS15355 - 66175..66300 (+) 126 WP_009924216.1 hypothetical protein -
  G8O86_RS00310 - 66311..66475 (+) 165 WP_003727776.1 hypothetical protein -
  G8O86_RS00315 - 66494..66928 (+) 435 WP_033918287.1 ArpU family phage packaging/lysis transcriptional regulator -
  G8O86_RS00320 - 67110..67742 (+) 633 WP_015987428.1 hypothetical protein -
  G8O86_RS00325 - 67823..68050 (+) 228 WP_012951944.1 hypothetical protein -
  G8O86_RS00330 terS 68090..68830 (+) 741 WP_015987406.1 phage terminase small subunit -
  G8O86_RS00335 - 68823..70142 (+) 1320 WP_033918288.1 PBSX family phage terminase large subunit -
  G8O86_RS00340 - 70157..71713 (+) 1557 WP_015987416.1 hypothetical protein -
  G8O86_RS00345 - 71718..72758 (+) 1041 WP_015987421.1 phage minor capsid protein -
  G8O86_RS00350 - 72854..73408 (+) 555 WP_015987425.1 hypothetical protein -
  G8O86_RS00355 - 73431..74303 (+) 873 WP_015987427.1 F420-dependent oxidoreductase -
  G8O86_RS00360 - 74317..74484 (+) 168 WP_172425835.1 hypothetical protein -
  G8O86_RS00365 - 74485..74838 (+) 354 WP_012951937.1 hypothetical protein -
  G8O86_RS00370 - 74838..75203 (+) 366 WP_015987430.1 hypothetical protein -
  G8O86_RS00375 - 75193..75510 (+) 318 WP_015987407.1 HK97 gp10 family phage protein -
  G8O86_RS00380 - 75507..75878 (+) 372 WP_003725064.1 hypothetical protein -
  G8O86_RS00385 - 75883..76569 (+) 687 WP_015987408.1 phage tail tube protein -
  G8O86_RS00390 - 76625..77056 (+) 432 WP_015987409.1 hypothetical protein -
  G8O86_RS00395 - 77089..77364 (+) 276 WP_015987410.1 Gp15 family bacteriophage protein -
  G8O86_RS00400 - 77369..82168 (+) 4800 WP_058876468.1 phage tail tape measure protein -
  G8O86_RS00405 - 82165..83733 (+) 1569 WP_015987412.1 distal tail protein Dit -
  G8O86_RS00410 - 83746..85908 (+) 2163 WP_015987413.1 phage tail spike protein -
  G8O86_RS00415 - 85959..86264 (+) 306 WP_003733957.1 hypothetical protein -
  G8O86_RS00420 - 86261..86545 (+) 285 WP_223196555.1 phage holin -
  G8O86_RS00425 - 86545..87252 (+) 708 WP_003723293.1 M15 family metallopeptidase -
  G8O86_RS00430 - 87491..87667 (+) 177 WP_003733956.1 hypothetical protein -
  G8O86_RS00435 - 87654..88253 (+) 600 WP_003723292.1 N-acetyltransferase -
  G8O86_RS00440 acrIIA1 88325..88774 (-) 450 WP_012581438.1 anti-CRISPR protein AcrIIA1 -
  G8O86_RS00445 - 88779..89129 (-) 351 WP_023553814.1 AcrIIA2 family anti-CRISPR protein -
  G8O86_RS00450 acrIIA3 89161..89538 (-) 378 WP_023553812.1 anti-CRISPR protein AcrIIA3 -
  G8O86_RS00455 - 89827..90060 (+) 234 WP_009924650.1 hypothetical protein -
  G8O86_RS00460 - 90057..90260 (+) 204 WP_003731279.1 hypothetical protein -
  G8O86_RS00465 - 90512..90847 (-) 336 WP_020246724.1 DUF898 family protein -
  G8O86_RS00470 - 91074..91472 (+) 399 WP_009924653.1 hypothetical protein -

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 17837.65 Da        Isoelectric Point: 4.9909

>NTDB_id=570586 G8O86_RS00290 WP_003722552.1 64763..65245(+) (ssbA) [Listeria monocytogenes strain 2HF15]
MMNRVVLVGRLTKDPDLRYTPAGVAVATFTLAVNRTFTNQNGEREADFINCVVWRKPAENVANFLKKGSMAGVDGRVQTR
NYEDNDGKRVFVTEVVAESVQFLEPKNNNVEGATSNNYQNKANYSNNNQTSSYRADTSQKSDSFASEGKPIDINEDDLPF

Nucleotide


Download         Length: 483 bp        

>NTDB_id=570586 G8O86_RS00290 WP_003722552.1 64763..65245(+) (ssbA) [Listeria monocytogenes strain 2HF15]
ATGATGAATCGTGTAGTACTTGTAGGACGATTAACAAAAGATCCGGATTTACGTTACACTCCAGCAGGCGTAGCAGTCGC
GACTTTTACATTAGCAGTAAATCGTACATTCACTAATCAAAACGGAGAACGAGAAGCAGATTTCATTAATTGTGTTGTTT
GGCGCAAACCAGCAGAAAACGTTGCTAATTTCTTGAAGAAAGGAAGCATGGCGGGCGTTGATGGACGTGTTCAAACTCGT
AATTATGAGGATAACGACGGTAAACGTGTTTTCGTTACTGAGGTAGTTGCTGAATCAGTTCAATTCTTAGAACCTAAAAA
TAACAACGTAGAAGGTGCTACATCGAATAATTACCAAAACAAGGCTAATTATTCAAATAACAATCAAACAAGCTCATATC
GAGCGGATACGAGTCAGAAGAGCGATTCATTTGCAAGTGAAGGTAAGCCGATTGATATTAATGAAGATGATTTGCCATTT
TGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3T2EU60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

65.116

100

0.7
  ssb Latilactobacillus sakei subsp. sakei 23K

55.294

100

0.587
  ssbB Bacillus subtilis subsp. subtilis str. 168

63.208

66.25

0.419