Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MMZ68_RS30105 Genome accession   NZ_CP093028
Coordinates   6416316..6416939 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain H05     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6411316..6421939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MMZ68_RS30085 (MMZ68_30085) aroB 6411911..6413017 (-) 1107 WP_019484454.1 3-dehydroquinate synthase -
  MMZ68_RS30090 (MMZ68_30090) aroK 6413067..6413585 (-) 519 WP_003095833.1 shikimate kinase AroK -
  MMZ68_RS30095 (MMZ68_30095) pilQ 6413597..6415741 (-) 2145 WP_015503770.1 type 4a pilus secretin PilQ Machinery gene
  MMZ68_RS30100 (MMZ68_30100) comL/pilP 6415795..6416319 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  MMZ68_RS30105 (MMZ68_30105) pilO 6416316..6416939 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  MMZ68_RS30110 (MMZ68_30110) pilN 6416936..6417532 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  MMZ68_RS30115 (MMZ68_30115) pilM 6417532..6418596 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  MMZ68_RS30120 (MMZ68_30120) - 6418781..6421252 (+) 2472 WP_019484455.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=570020 MMZ68_RS30105 WP_003103268.1 6416316..6416939(-) (pilO) [Pseudomonas aeruginosa strain H05]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=570020 MMZ68_RS30105 WP_003103268.1 6416316..6416939(-) (pilO) [Pseudomonas aeruginosa strain H05]
ATGAGTCTGGCCAGTTCCCTGGAAAGTTTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTCC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401