Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KJP35_RS08350 Genome accession   NZ_CP075563
Coordinates   1665012..1665986 (+) Length   324 a.a.
NCBI ID   WP_069995863.1    Uniprot ID   -
Organism   Staphylococcus aureus strain Sta2021027     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1660012..1670986
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP35_RS08315 (KJP35_08265) rpmG 1660472..1660621 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  KJP35_RS08320 (KJP35_08270) - 1660830..1661360 (+) 531 WP_072384884.1 5-formyltetrahydrofolate cyclo-ligase -
  KJP35_RS08325 (KJP35_08275) - 1661381..1662844 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  KJP35_RS08330 (KJP35_08280) - 1662825..1663028 (+) 204 WP_000087561.1 YqgQ family protein -
  KJP35_RS08335 (KJP35_08285) - 1663025..1664011 (+) 987 WP_000161314.1 ROK family glucokinase -
  KJP35_RS08340 (KJP35_08290) - 1664011..1664340 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KJP35_RS08345 (KJP35_08295) - 1664337..1664960 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KJP35_RS08350 (KJP35_08300) comGA 1665012..1665986 (+) 975 WP_069995863.1 competence type IV pilus ATPase ComGA Machinery gene
  KJP35_RS08355 (KJP35_08305) comGB 1665958..1667028 (+) 1071 WP_045176802.1 competence type IV pilus assembly protein ComGB Machinery gene
  KJP35_RS08360 (KJP35_08310) comGC 1667042..1667353 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KJP35_RS08365 (KJP35_08315) comGD 1667331..1667777 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  KJP35_RS08370 (KJP35_08320) comGE 1667764..1668063 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KJP35_RS08375 (KJP35_08325) comGF 1667981..1668478 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  KJP35_RS08380 (KJP35_08330) - 1668575..1668721 (+) 147 WP_001789879.1 hypothetical protein -
  KJP35_RS08385 (KJP35_08335) - 1668711..1669235 (+) 525 WP_001015121.1 shikimate kinase -
  KJP35_RS08390 (KJP35_08340) gcvT 1669394..1670485 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36824.93 Da        Isoelectric Point: 8.8508

>NTDB_id=569648 KJP35_RS08350 WP_069995863.1 1665012..1665986(+) (comGA) [Staphylococcus aureus strain Sta2021027]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLINKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=569648 KJP35_RS08350 WP_069995863.1 1665012..1665986(+) (comGA) [Staphylococcus aureus strain Sta2021027]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATTAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991