Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   KJP35_RS08355 Genome accession   NZ_CP075563
Coordinates   1665958..1667028 (+) Length   356 a.a.
NCBI ID   WP_045176802.1    Uniprot ID   -
Organism   Staphylococcus aureus strain Sta2021027     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1660958..1672028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJP35_RS08325 (KJP35_08275) - 1661381..1662844 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  KJP35_RS08330 (KJP35_08280) - 1662825..1663028 (+) 204 WP_000087561.1 YqgQ family protein -
  KJP35_RS08335 (KJP35_08285) - 1663025..1664011 (+) 987 WP_000161314.1 ROK family glucokinase -
  KJP35_RS08340 (KJP35_08290) - 1664011..1664340 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KJP35_RS08345 (KJP35_08295) - 1664337..1664960 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KJP35_RS08350 (KJP35_08300) comGA 1665012..1665986 (+) 975 WP_069995863.1 competence type IV pilus ATPase ComGA Machinery gene
  KJP35_RS08355 (KJP35_08305) comGB 1665958..1667028 (+) 1071 WP_045176802.1 competence type IV pilus assembly protein ComGB Machinery gene
  KJP35_RS08360 (KJP35_08310) comGC 1667042..1667353 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KJP35_RS08365 (KJP35_08315) comGD 1667331..1667777 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  KJP35_RS08370 (KJP35_08320) comGE 1667764..1668063 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KJP35_RS08375 (KJP35_08325) comGF 1667981..1668478 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  KJP35_RS08380 (KJP35_08330) - 1668575..1668721 (+) 147 WP_001789879.1 hypothetical protein -
  KJP35_RS08385 (KJP35_08335) - 1668711..1669235 (+) 525 WP_001015121.1 shikimate kinase -
  KJP35_RS08390 (KJP35_08340) gcvT 1669394..1670485 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  KJP35_RS08395 (KJP35_08345) gcvPA 1670505..1671851 (+) 1347 WP_045177127.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41456.85 Da        Isoelectric Point: 10.0606

>NTDB_id=569649 KJP35_RS08355 WP_045176802.1 1665958..1667028(+) (comGB) [Staphylococcus aureus strain Sta2021027]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPHILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=569649 KJP35_RS08355 WP_045176802.1 1665958..1667028(+) (comGB) [Staphylococcus aureus strain Sta2021027]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATATCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCATATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997