Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdL   Type   Machinery gene
Locus tag   KHC33_RS00875 Genome accession   NZ_CP075546
Coordinates   168645..170486 (+) Length   613 a.a.
NCBI ID   WP_214419922.1    Uniprot ID   -
Organism   Methanospirillum purgamenti strain J.3.6.1-F.2.7.3     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 163645..175486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHC33_RS00845 (KHC33_00845) - 164487..165941 (+) 1455 WP_214419916.1 preprotein translocase subunit SecD -
  KHC33_RS00850 (KHC33_00850) - 165994..166266 (+) 273 WP_214419917.1 thioredoxin family protein -
  KHC33_RS00855 (KHC33_00855) npdG 166268..166918 (+) 651 WP_214419918.1 NADPH-dependent F420 reductase -
  KHC33_RS00860 (KHC33_00860) - 166926..167462 (+) 537 WP_214419919.1 aldolase -
  KHC33_RS00865 (KHC33_00865) - 167465..167842 (+) 378 WP_214419920.1 hypothetical protein -
  KHC33_RS00870 (KHC33_00870) - 167845..168468 (-) 624 WP_214419921.1 RAD55 family ATPase -
  KHC33_RS00875 (KHC33_00875) epdL 168645..170486 (+) 1842 WP_214419922.1 type II/IV secretion system ATPase subunit Machinery gene
  KHC33_RS00880 (KHC33_00880) epdJ 170473..171408 (+) 936 WP_214419923.1 type II secretion system F family protein Machinery gene
  KHC33_RS00885 (KHC33_00885) epdK 171411..172403 (+) 993 WP_214419924.1 type II secretion system F family protein Machinery gene
  KHC33_RS00890 (KHC33_00890) - 172393..174435 (-) 2043 WP_214419925.1 CHASE4 domain-containing protein -

Sequence

Protein


Download         Length: 613 a.a.        Molecular weight: 69323.07 Da        Isoelectric Point: 6.9785

>NTDB_id=569412 KHC33_RS00875 WP_214419922.1 168645..170486(+) (epdL) [Methanospirillum purgamenti strain J.3.6.1-F.2.7.3]
MKVKVGSFKKQVRASNDKGKPAGEPSLQDTPPKEKVSRLSALSGKKKEKTGKTNKPASLRRLGKKDEKDQSVSGTGSGES
KKKGSLFGTIGKIAPLAKKREQYDPKVHGMMVDLTYVPPPGTEEIEVYPVNPPFAYIRITYNYTTHEYLYEVVEPVYSEA
EKKLLDELKKGIFERVNVNTRQISRETAGETLESMMNEVLADYAINLDPLTYEKIHYHLKKDFLGDGLVDPVMHDRFIED
ISCDGIGRPLFVYHSRYESIESNLFYSDNPELDSFVTKLAQRAGKHISVADPMLDATMQDGSRIQMTLGTEVTAHGSTFT
IRKFKDEPITPTDLIEWGTFSPLSIAFLWLAVENGKSCIFAGGTASGKTTSLNAISLFIPPLAKIVTLEDTRELKLPHAN
WIPSVTRESFDVSGKGEIDMYELLKAALRQRPEYLIVGEVRGREALTLFQAMSTGHVTYSTMHADSVSSAVHRLENPPIS
VPRNMLSALNLVCVQVQARVGGQRIRRNKQIIEVLDIDPRTNELITNEVFRWRQATDEISYSGKSFILEEIMESHGWDEE
RIRRDLLQRQEVLEWMREQKIRDFRDVSKIVVSYFRDSETLMEQIRSGGNEFL

Nucleotide


Download         Length: 1842 bp        

>NTDB_id=569412 KHC33_RS00875 WP_214419922.1 168645..170486(+) (epdL) [Methanospirillum purgamenti strain J.3.6.1-F.2.7.3]
ATGAAAGTAAAGGTGGGGAGTTTTAAAAAACAGGTTCGTGCATCGAATGATAAGGGAAAACCTGCCGGAGAGCCATCTTT
ACAAGATACTCCACCTAAGGAAAAAGTTTCCCGACTGTCTGCCCTGTCTGGCAAAAAGAAAGAAAAAACTGGAAAAACCA
ATAAACCAGCAAGTTTACGCCGGCTTGGGAAGAAGGATGAAAAAGATCAGTCCGTTTCTGGTACAGGATCTGGGGAATCG
AAAAAGAAGGGTTCACTATTTGGAACAATTGGAAAAATAGCACCTCTGGCAAAAAAACGTGAGCAATATGATCCCAAGGT
CCATGGGATGATGGTTGACCTCACGTATGTGCCTCCTCCCGGGACTGAAGAGATAGAGGTCTATCCGGTTAATCCTCCTT
TTGCATATATCCGGATTACGTACAACTATACCACGCATGAGTACCTGTACGAAGTTGTTGAACCGGTATATTCAGAAGCA
GAAAAGAAGCTTCTCGATGAGCTTAAAAAAGGAATATTTGAACGGGTGAATGTAAACACCCGACAGATTTCACGTGAGAC
TGCGGGGGAGACTCTTGAGTCCATGATGAATGAGGTGCTGGCAGATTATGCAATCAACCTGGACCCTCTGACCTATGAGA
AGATCCATTATCACCTGAAAAAAGACTTTCTTGGGGATGGACTTGTTGATCCGGTGATGCATGATCGGTTTATTGAAGAT
ATCTCCTGTGACGGTATCGGCAGACCTCTTTTTGTATATCATTCGAGATACGAATCAATAGAGTCTAATCTCTTCTATTC
TGATAATCCAGAACTTGATTCGTTTGTCACTAAGCTTGCTCAGCGTGCCGGAAAACACATCTCAGTTGCAGATCCGATGC
TGGATGCAACAATGCAGGATGGATCCCGTATTCAGATGACACTCGGGACCGAAGTAACAGCACATGGGTCTACTTTTACC
ATCCGGAAATTCAAAGATGAACCGATCACCCCGACTGACCTGATCGAATGGGGTACCTTTTCTCCTCTCTCGATAGCATT
CCTCTGGCTTGCTGTTGAAAACGGGAAGTCATGCATCTTTGCCGGTGGGACAGCGTCAGGAAAGACAACCTCACTGAATG
CTATCTCCCTATTTATTCCACCCTTGGCAAAGATAGTCACACTTGAGGATACCAGGGAACTGAAGCTTCCCCATGCCAAT
TGGATTCCAAGTGTCACCCGCGAGTCCTTTGATGTGAGCGGTAAAGGGGAGATCGATATGTATGAACTCTTAAAAGCTGC
TCTTCGTCAGCGTCCTGAGTATCTGATCGTCGGTGAAGTCCGTGGTCGTGAAGCACTCACTCTCTTTCAGGCGATGTCAA
CCGGGCATGTGACATATTCCACAATGCACGCAGATTCAGTATCCTCAGCAGTCCATCGTCTGGAAAATCCTCCCATCTCA
GTTCCAAGAAACATGCTTTCTGCCTTAAACCTGGTTTGTGTGCAGGTCCAGGCACGGGTCGGCGGTCAGCGCATCCGGCG
TAACAAACAGATTATCGAGGTCCTGGATATTGATCCCAGAACCAATGAACTGATCACAAATGAAGTATTCAGATGGAGGC
AGGCAACCGATGAGATCTCATACTCTGGCAAGTCGTTTATTCTGGAAGAGATCATGGAGTCACATGGGTGGGATGAGGAA
AGAATTCGTCGTGACCTTCTCCAGCGGCAGGAAGTACTTGAGTGGATGCGTGAACAGAAAATCCGCGACTTTCGTGATGT
GAGCAAAATTGTTGTCTCGTACTTCAGGGATTCCGAGACCTTAATGGAACAGATCCGGTCTGGTGGCAATGAATTCTTAT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(238-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdL Methanoculleus thermophilus strain DSM 2373

71.893

85.318

0.613