Detailed information    

experimental Experimentally validated

Overview

Name   epdL   Type   Machinery gene
Locus tag   F7686_RS01490 Genome accession   NZ_FNFT01000001
Coordinates   289155..291338 (+) Length   727 a.a.
NCBI ID   WP_224732727.1    Uniprot ID   -
Organism   Methanoculleus thermophilus strain DSM 2373     
Function   DNA uptake   
DNA binding and uptake

Function

The M. thermophilus ΔepdJKL mutant was unable to take up extracellular DNA.


Genomic Context

Location: 284155..296338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7686_RS01470 (SAMN04488571_101304) - 284897..286780 (-) 1884 WP_066956416.1 PINc/VapC family ATPase -
  F7686_RS01475 (SAMN04488571_101305) hisI 286804..287169 (-) 366 WP_066956419.1 phosphoribosyl-AMP cyclohydrolase -
  F7686_RS01480 (SAMN04488571_101306) - 287176..287976 (-) 801 WP_066956422.1 A24 family peptidase C-terminal domain-containing protein -
  F7686_RS01485 (SAMN04488571_101307) - 288332..288970 (+) 639 WP_066956425.1 hypothetical protein -
  F7686_RS01490 (SAMN04488571_101308) epdL 289155..291338 (+) 2184 WP_224732727.1 type II/IV secretion system ATPase subunit Machinery gene
  F7686_RS01495 (SAMN04488571_101309) epdJ 291331..292254 (+) 924 WP_066956430.1 type II secretion system F family protein Machinery gene
  F7686_RS01500 (SAMN04488571_101310) epdK 292256..293308 (+) 1053 WP_066956433.1 type II secretion system F family protein Machinery gene
  F7686_RS01505 (SAMN04488571_101311) - 293383..294876 (-) 1494 WP_066956436.1 cobyric acid synthase -

Sequence

Protein


Download         Length: 727 a.a.        Molecular weight: 81969.88 Da        Isoelectric Point: 4.9926

>NTDB_id=64 F7686_RS01490 WP_224732727.1 289155..291338(+) (epdL) [Methanoculleus thermophilus strain DSM 2373]
MREHSPGPVLRGADIRSIIGSVMSSEDTRGRRTTADEDPVRALLNRLERSGRETDKERTVTPSPMSSDTGPKAAPSSPGH
RDPFGILNRVRGMKEAGEAGEMEAEGPGSEDLPDGVISVEELGSLADLILPKSATFAVEELSINRNEHNFDFVDNARVVS
EFDDLFSQALSSTAFAEAAAEVASPSEEVPKPRFGFLQKLRPLRPEMEIEEYNPAVHGPLLDLSMRPSPGVEEIELYPVN
EPYAYVRVIYDSATHEYTYCVLEPELTPAERELFLEIKERLFETLNITTRDLTREAARKALRDAANRIIADYGIHLDAVG
REKVLYHVEKEFLGDGLIDPVMHDKYIEDISCDGVNCPIFVYHTTYESMKTNLLYHDAAELDSFVAKLAQRAGKYISIAE
PMLDATMSDGSRIQMTLGSEVTAHGSTFTIRKFREEPITPTDLIEWHTFSPLGIAFIWLAVENGKSCIFAGGTASGKTTT
LNAISLFIPPLAKIVTLEDTRELKLPHPNWIPSITRDSFSQDGRGEIDMYELLRAALRQRPEYILVGEVRGREALTLFQA
MSTGHVTYATMHADSVASAVHRLENPPINVPRNMLSALSLMSIQVQARVGGQRIRRNKQLIEILDIDPRTNELITNEVFR
WHPATDEIRYSGKSYILEQIMEDRGWSEERMQEELKRRQEVLEWMRIKKIRHFRDVSKVLVSYFRDPEGVIERVRSDLYG
GMVRQHE

Nucleotide


Download         Length: 2184 bp        

>NTDB_id=64 F7686_RS01490 WP_224732727.1 289155..291338(+) (epdL) [Methanoculleus thermophilus strain DSM 2373]
ATGCGTGAGCATTCTCCTGGCCCGGTCTTGAGAGGGGCCGATATCCGTTCAATCATCGGCTCGGTGATGAGTTCAGAAGA
TACCAGGGGGCGGCGCACAACTGCTGATGAGGATCCTGTCCGGGCACTCCTCAACCGGCTCGAGAGGTCGGGGAGGGAGA
CTGATAAGGAGCGTACGGTCACCCCTTCGCCGATGAGCAGTGATACAGGGCCGAAGGCTGCACCCTCTTCACCCGGGCAT
CGTGATCCCTTTGGTATCCTCAACCGGGTGAGAGGGATGAAAGAGGCCGGCGAGGCCGGGGAGATGGAGGCTGAAGGGCC
CGGGTCCGAAGATCTCCCCGATGGGGTGATATCGGTCGAGGAACTCGGGAGCCTGGCAGACCTGATCCTCCCGAAGAGTG
CGACGTTTGCTGTCGAAGAACTGAGTATCAACCGCAACGAGCACAATTTCGACTTCGTCGACAACGCCCGGGTCGTCTCG
GAGTTCGATGACCTCTTCTCCCAGGCACTCTCCTCCACCGCGTTTGCCGAGGCCGCGGCGGAGGTGGCGTCTCCCTCCGA
AGAGGTTCCAAAGCCCCGGTTCGGGTTCCTCCAGAAGCTCCGTCCACTTCGTCCAGAGATGGAGATCGAGGAGTACAACC
CTGCCGTCCACGGGCCACTTCTCGATCTTTCAATGCGCCCCTCCCCGGGAGTGGAAGAGATCGAACTCTACCCGGTAAAC
GAACCATACGCCTACGTCAGGGTCATCTACGACAGCGCAACCCATGAGTACACCTACTGTGTGCTTGAGCCTGAACTCAC
GCCTGCGGAGCGGGAACTCTTCCTGGAGATCAAGGAGCGTCTCTTTGAGACGCTCAATATCACCACCCGTGACCTCACAC
GCGAGGCGGCACGCAAAGCGCTTCGCGACGCGGCAAACAGAATCATCGCCGATTACGGGATCCATCTTGATGCGGTGGGG
CGGGAGAAGGTTCTGTATCACGTGGAGAAGGAGTTCCTCGGCGACGGATTGATCGACCCGGTGATGCACGACAAGTACAT
CGAGGATATCTCGTGCGACGGCGTGAACTGCCCAATCTTCGTCTACCACACCACATACGAATCGATGAAGACGAACCTTC
TCTACCACGACGCGGCGGAACTCGACTCGTTTGTTGCCAAACTCGCGCAGCGGGCCGGGAAGTACATCTCGATTGCTGAA
CCGATGCTGGACGCCACGATGAGCGATGGGTCGCGTATCCAGATGACGCTCGGCTCGGAGGTGACCGCCCACGGTTCAAC
GTTCACGATCCGGAAGTTCCGCGAAGAGCCGATCACGCCGACGGATCTCATCGAGTGGCACACCTTCTCACCGCTCGGGA
TCGCCTTCATCTGGCTCGCGGTCGAGAACGGCAAGTCCTGCATATTTGCAGGCGGTACGGCGTCCGGAAAGACCACGACC
CTGAACGCTATATCGCTCTTCATCCCGCCGCTTGCAAAGATCGTCACCCTCGAGGATACCCGTGAGTTGAAACTCCCTCA
CCCGAACTGGATCCCGAGCATCACCCGAGACTCGTTCTCGCAGGACGGGCGGGGCGAGATCGATATGTACGAACTCCTGC
GTGCCGCCCTCCGGCAGCGCCCGGAGTATATTCTGGTCGGCGAGGTCCGTGGCCGTGAGGCCTTGACCCTCTTTCAGGCG
ATGAGCACCGGGCACGTCACCTACGCGACGATGCACGCCGACTCGGTAGCGAGTGCCGTCCACCGTCTGGAGAACCCGCC
GATCAACGTGCCGAGGAACATGCTCTCTGCCCTCTCCCTGATGTCCATCCAGGTGCAGGCCCGGGTGGGGGGCCAGCGGA
TCCGGCGGAACAAGCAGCTCATCGAGATCCTGGATATCGATCCCCGGACGAACGAACTGATCACGAACGAGGTCTTCCGG
TGGCATCCCGCGACCGACGAGATTCGTTACTCGGGCAAATCCTACATCCTCGAGCAGATCATGGAGGACCGGGGATGGAG
CGAGGAGCGGATGCAGGAAGAACTGAAGCGGCGTCAGGAGGTGCTTGAATGGATGCGCATCAAGAAGATCCGGCATTTCC
GCGATGTAAGCAAGGTCCTTGTCTCCTACTTCCGCGATCCGGAGGGGGTCATCGAACGCGTCCGTTCTGACCTTTATGGT
GGGATGGTGAGGCAGCATGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(346-598)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2020) Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea. Journal of Bacteriology 202(21):e00355-20. [PMID: 32817089]