Detailed information    

experimental Experimentally validated

Overview

Name   epdK   Type   Machinery gene
Locus tag   F7686_RS01500 Genome accession   NZ_FNFT01000001
Coordinates   292256..293308 (+) Length   350 a.a.
NCBI ID   WP_066956433.1    Uniprot ID   A0A1G8XB24
Organism   Methanoculleus thermophilus strain DSM 2373     
Function   DNA uptake   
DNA binding and uptake

Function

The M. thermophilus ΔepdJKL mutant was unable to take up extracellular DNA.


Genomic Context

Location: 287256..298308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7686_RS01485 (SAMN04488571_101307) - 288332..288970 (+) 639 WP_066956425.1 hypothetical protein -
  F7686_RS01490 (SAMN04488571_101308) epdL 289155..291338 (+) 2184 WP_224732727.1 type II/IV secretion system ATPase subunit Machinery gene
  F7686_RS01495 (SAMN04488571_101309) epdJ 291331..292254 (+) 924 WP_066956430.1 type II secretion system F family protein Machinery gene
  F7686_RS01500 (SAMN04488571_101310) epdK 292256..293308 (+) 1053 WP_066956433.1 type II secretion system F family protein Machinery gene
  F7686_RS01505 (SAMN04488571_101311) - 293383..294876 (-) 1494 WP_066956436.1 cobyric acid synthase -
  F7686_RS01510 (SAMN04488571_101312) - 294936..296582 (+) 1647 WP_066956985.1 adenosylcobalamin-dependent ribonucleoside-diphosphate reductase -
  F7686_RS01515 (SAMN04488571_101313) yciH 296624..296929 (+) 306 WP_066956439.1 stress response translation initiation inhibitor YciH -
  F7686_RS01520 (SAMN04488571_101314) - 297152..297490 (+) 339 WP_066956442.1 type II secretion system protein E -
  F7686_RS01525 - 297496..297756 (-) 261 WP_066956446.1 hypothetical protein -
  F7686_RS01530 (SAMN04488571_101316) - 297749..298246 (-) 498 WP_150468679.1 YkgJ family cysteine cluster protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38590.96 Da        Isoelectric Point: 5.0549

>NTDB_id=63 F7686_RS01500 WP_066956433.1 292256..293308(+) (epdK) [Methanoculleus thermophilus strain DSM 2373]
MGFKEIIDDFLNRLPGQDRTTGGAAADALVSESFEEREEEIFGRIENWRKYQEGFYRFLKHPLRVMAEEPLTVLFISIPA
ALLLFAGGFMSLVLSYGVGVLFSTTMIDDVFVFSLLIAIVPLAILDLKESLRVSSIEASLPNFFRDVAGMNDSGMTLPHA
IHIVSEGEYGALTPHIRKLDTEMSWGVPFVEAIRRFGKAVNTPLTERSVDLIAHASSAGGDVSEVLRAAAHDAYEFFNLK
MERRNGMMIYMIIIVMSFFVFLFVIGVLSSTFLTTMAEAGTAVAASGSTQTFMGTVDLFLYNRLFCHAALIQGFFSGLGA
GVMGEGRVLAGLKYSAIMVLIAWIAFRFFI

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=63 F7686_RS01500 WP_066956433.1 292256..293308(+) (epdK) [Methanoculleus thermophilus strain DSM 2373]
ATGGGGTTCAAAGAGATTATCGATGACTTTCTGAACCGATTGCCGGGCCAGGACCGAACGACGGGCGGCGCGGCGGCGGA
CGCCCTGGTGTCCGAGTCGTTTGAGGAGCGGGAAGAGGAGATCTTCGGCCGGATAGAGAACTGGCGGAAGTATCAGGAAG
GGTTTTACCGGTTCCTCAAGCACCCGCTTAGAGTTATGGCGGAAGAACCCCTCACCGTCCTCTTCATCTCCATCCCAGCT
GCCCTCCTCCTCTTTGCCGGCGGATTTATGAGCCTGGTCCTCTCCTACGGCGTCGGCGTCCTCTTTTCGACGACGATGAT
CGACGATGTCTTTGTCTTCTCGCTCCTCATCGCCATCGTGCCGCTTGCAATCCTCGACCTCAAGGAGTCGCTGCGGGTAT
CAAGCATCGAGGCATCCCTCCCGAACTTCTTCCGGGATGTGGCCGGGATGAACGACTCGGGCATGACCCTCCCGCATGCA
ATCCATATCGTCTCGGAGGGGGAGTACGGGGCGCTCACGCCGCACATCCGTAAACTCGACACCGAGATGTCCTGGGGCGT
CCCGTTCGTCGAGGCCATTCGCCGATTCGGGAAGGCCGTGAACACCCCGCTCACCGAACGGAGCGTCGACCTGATCGCCC
ACGCGAGTTCGGCCGGCGGCGACGTGAGCGAGGTGCTGCGGGCTGCGGCACACGATGCCTACGAGTTCTTCAACCTGAAG
ATGGAGCGGAGAAACGGGATGATGATCTACATGATCATCATCGTTATGTCGTTCTTCGTCTTCCTCTTCGTCATCGGGGT
GCTCTCAAGCACGTTCCTCACCACGATGGCGGAGGCGGGGACGGCGGTTGCGGCCTCGGGTTCGACCCAGACATTCATGG
GGACCGTAGACCTCTTCCTCTACAACCGCCTCTTCTGTCACGCCGCACTGATCCAGGGGTTCTTCTCGGGACTCGGGGCC
GGGGTCATGGGCGAGGGCCGGGTGCTCGCCGGTCTGAAGTACTCGGCGATCATGGTGCTGATCGCCTGGATCGCGTTCCG
GTTCTTTATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(143-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G8XB24

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Dallas R Fonseca et al. (2020) Type IV-Like Pili Facilitate Transformation in Naturally Competent Archaea. Journal of Bacteriology 202(21):e00355-20. [PMID: 32817089]