Detailed information    

insolico Bioinformatically predicted

Overview

Name   epdJ   Type   Machinery gene
Locus tag   KHC33_RS00880 Genome accession   NZ_CP075546
Coordinates   170473..171408 (+) Length   311 a.a.
NCBI ID   WP_214419923.1    Uniprot ID   -
Organism   Methanospirillum purgamenti strain J.3.6.1-F.2.7.3     
Function   DNA uptake (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 165473..176408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHC33_RS00850 (KHC33_00850) - 165994..166266 (+) 273 WP_214419917.1 thioredoxin family protein -
  KHC33_RS00855 (KHC33_00855) npdG 166268..166918 (+) 651 WP_214419918.1 NADPH-dependent F420 reductase -
  KHC33_RS00860 (KHC33_00860) - 166926..167462 (+) 537 WP_214419919.1 aldolase -
  KHC33_RS00865 (KHC33_00865) - 167465..167842 (+) 378 WP_214419920.1 hypothetical protein -
  KHC33_RS00870 (KHC33_00870) - 167845..168468 (-) 624 WP_214419921.1 RAD55 family ATPase -
  KHC33_RS00875 (KHC33_00875) epdL 168645..170486 (+) 1842 WP_214419922.1 type II/IV secretion system ATPase subunit Machinery gene
  KHC33_RS00880 (KHC33_00880) epdJ 170473..171408 (+) 936 WP_214419923.1 type II secretion system F family protein Machinery gene
  KHC33_RS00885 (KHC33_00885) epdK 171411..172403 (+) 993 WP_214419924.1 type II secretion system F family protein Machinery gene
  KHC33_RS00890 (KHC33_00890) - 172393..174435 (-) 2043 WP_214419925.1 CHASE4 domain-containing protein -
  KHC33_RS00895 (KHC33_00895) - 174583..176040 (-) 1458 WP_214419926.1 response regulator -

Sequence

Protein


Download         Length: 311 a.a.        Molecular weight: 34350.10 Da        Isoelectric Point: 5.0663

>NTDB_id=569413 KHC33_RS00880 WP_214419923.1 170473..171408(+) (epdJ) [Methanospirillum purgamenti strain J.3.6.1-F.2.7.3]
MNSYERFCFSLFGSRMSGKREKYYELIENLKSARMGITFEAYLSTAVFTSVLVGIIGSILIALLVYLLNVPALISFRGNV
PTDIAATINQFQGYYLIVGTLIAGIVSFVVLYGTTFLIYLVYPSVVAGGRRRNIETTLPYAINYLAAMSTAGIPPAEVFR
QLGNSTIYGESSVEARYIILEVDVFGHDLIEALKNVSMSTPSPRMRDFLQGCIGSVAAGSNISDFFRTKAEQYTGENRQT
QKQFLDTLALIAESYVTALVAGTLFMILIQSIMAILSGESTPLFLYIIIYLIIPFGSVMFVVLIDSMTPEW

Nucleotide


Download         Length: 936 bp        

>NTDB_id=569413 KHC33_RS00880 WP_214419923.1 170473..171408(+) (epdJ) [Methanospirillum purgamenti strain J.3.6.1-F.2.7.3]
ATGAATTCTTATGAGCGGTTCTGCTTCTCCCTGTTTGGAAGCCGGATGTCAGGCAAACGGGAGAAATATTATGAACTCAT
TGAGAATCTCAAGTCTGCCAGAATGGGTATTACTTTTGAAGCATACCTTTCTACTGCTGTATTCACCTCTGTTCTTGTCG
GAATTATTGGCTCAATCCTGATTGCCCTTCTTGTGTACCTCCTGAACGTCCCGGCACTGATATCTTTTCGGGGTAATGTT
CCCACGGATATTGCAGCGACTATCAACCAGTTCCAGGGATACTACCTCATAGTGGGGACACTTATTGCTGGCATCGTCTC
TTTTGTGGTGCTGTATGGGACGACATTTCTCATCTATCTGGTTTATCCTTCTGTAGTTGCTGGGGGAAGAAGACGCAATA
TCGAGACAACCCTTCCATATGCTATCAACTATCTTGCTGCAATGTCTACCGCAGGCATCCCTCCGGCAGAGGTTTTCCGG
CAACTGGGGAACTCGACGATATATGGTGAGTCTTCGGTTGAAGCTCGGTATATCATCCTTGAGGTGGATGTGTTTGGGCA
TGATCTTATCGAAGCATTGAAAAATGTCTCCATGAGTACACCAAGTCCGCGTATGCGTGACTTTCTCCAGGGGTGTATCG
GAAGTGTTGCAGCGGGAAGCAACATCTCTGATTTTTTCAGGACAAAAGCAGAACAGTATACCGGCGAAAACAGGCAGACA
CAGAAACAATTTCTTGATACCCTCGCACTGATAGCCGAGAGTTATGTAACCGCTCTTGTTGCCGGAACTCTGTTCATGAT
TCTTATTCAGTCTATCATGGCTATTCTGTCTGGAGAATCAACACCCTTGTTTCTGTATATTATCATCTATCTGATCATTC
CGTTTGGAAGTGTGATGTTTGTGGTTCTGATAGATTCGATGACGCCGGAGTGGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(142-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  epdJ Methanoculleus thermophilus strain DSM 2373

60.323

99.678

0.601