Detailed information NTG0568931

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KIL00_RS13825
• Genome accession: NZ_CP075344
• Coordinates: 2640131..2640748 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis strain A1 - Midalam
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2635131..2645748

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KIL00_RS13795 (KIL00_13795)	rpsT	2635434..2635700 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
KIL00_RS13800 (KIL00_13800)	holA	2635715..2636758 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
KIL00_RS13805 (KIL00_13805)	-	2636798..2636947 (-)	150	WP_003229985.1	hypothetical protein	-
KIL00_RS13810 (KIL00_13810)	yqzM	2636988..2637122 (+)	135	WP_003229983.1	YqzM family protein	-
KIL00_RS13815 (KIL00_13815)	comEC	2637162..2639491 (-)	2330	Protein_2683	DNA internalization-related competence protein ComEC/Rec2	-
KIL00_RS13820 (KIL00_13820)	comEB	2639495..2640064 (-)	570	WP_003229978.1	ComE operon protein 2	-
KIL00_RS13825 (KIL00_13825)	comEA	2640131..2640748 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
KIL00_RS13830 (KIL00_13830)	comER	2640832..2641653 (+)	822	WP_032726195.1	late competence protein ComER	-
KIL00_RS13835 (KIL00_13835)	yqeM	2641719..2642462 (-)	744	WP_069837641.1	class I SAM-dependent methyltransferase	-
KIL00_RS13840 (KIL00_13840)	rsfS	2642459..2642815 (-)	357	WP_014480315.1	ribosome silencing factor	-
KIL00_RS13845 (KIL00_13845)	yqeK	2642833..2643393 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KIL00_RS13850 (KIL00_13850)	nadD	2643383..2643952 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
KIL00_RS13855 (KIL00_13855)	yhbY	2643964..2644254 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
KIL00_RS13860 (KIL00_13860)	aroE	2644248..2645090 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=568931 KIL00_RS13825 WP_046160608.1 2640131..2640748(-) (comEA) [Bacillus subtilis subsp. subtilis strain A1 - Midalam]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=568931 KIL00_RS13825 WP_046160608.1 2640131..2640748(-) (comEA) [Bacillus subtilis subsp. subtilis strain A1 - Midalam]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376