Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   MK848_RS20425 Genome accession   NZ_CP092824
Coordinates   3957427..3958776 (-) Length   449 a.a.
NCBI ID   WP_009967069.1    Uniprot ID   O34358
Organism   Bacillus subtilis strain S1     
Function   degrading CSP; selective degradation of ComEA and ComEC (predicted from homology)   
Competence regulation

Genomic Context

Location: 3952427..3963776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK848_RS20405 (MK848_20360) mhqA 3952609..3953559 (+) 951 WP_014476561.1 ring-cleaving dioxygenase -
  MK848_RS20410 (MK848_20365) - 3953668..3953763 (+) 96 Protein_3960 hypothetical protein -
  MK848_RS20415 (MK848_20370) ykcB 3953776..3955926 (+) 2151 WP_015715746.1 glycosyltransferase family 39 protein -
  MK848_RS20420 (MK848_20375) gtcC 3955938..3956909 (+) 972 WP_015715747.1 glycosyltransferase family 2 protein -
  MK848_RS20425 (MK848_20380) htrA 3957427..3958776 (-) 1350 WP_009967069.1 serine protease HtrA Regulator
  MK848_RS20430 (MK848_20385) proG 3958945..3959763 (+) 819 WP_029727042.1 pyrroline-5-carboxylate reductase ProG -
  MK848_RS20435 (MK848_20390) dppA 3959892..3960716 (+) 825 WP_014479576.1 D-aminopeptidase DppA -
  MK848_RS20440 (MK848_20395) dppB 3960733..3961659 (+) 927 WP_003245446.1 dipeptide ABC transporter permease DppB -
  MK848_RS20445 (MK848_20400) dppC 3961665..3962627 (+) 963 WP_029727041.1 dipeptide ABC transporter permease DppC -
  MK848_RS20450 (MK848_20405) dppD 3962632..3963639 (+) 1008 WP_029727040.1 dipeptide ABC transporter ATP-binding subunit DppD -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 47714.85 Da        Isoelectric Point: 4.5990

>NTDB_id=568464 MK848_RS20425 WP_009967069.1 3957427..3958776(-) (htrA) [Bacillus subtilis strain S1]
MDNYRDENRTKGNENEVFLTKENDQSASYSARNVIHDQEKKKRGFGWFRPLLGGVIGGSLALGIYTFTPLGDHDSQDTAK
QSSSQQQTQSVTATSTSSESKKSSSSSSAFKSEDSSKISDMVEDLSPAIVGITNLQAQSNSSLFGSSSSDSSEDTESGSG
SGVIFKKENGKAYIITNNHVVEGASSLKVSLYDGTEVTAKLVGSDSLTDLAVLQISDDHVTKVANFGDSSDLRTGETVIA
IGDPLGKDLSRTVTQGIVSGVDRTVSMSTSAGETSINVIQTDAAINPGNSGGPLLNTDGKIVGINSMKISEDDVEGIGFA
IPSNDVKPIAEELLSKGQIERPYIGVSMLDLEQVPQNYQEGTLGLFGSQLNKGVYIREVASGSPAEKAGLKAEDIIIGLK
GKEIDTGSELRNILYKDAKIGDTVEVKILRNGKEMTKKIKLDQKEEKTS

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=568464 MK848_RS20425 WP_009967069.1 3957427..3958776(-) (htrA) [Bacillus subtilis strain S1]
ATGGATAACTATCGTGATGAAAACAGAACGAAAGGTAATGAGAATGAGGTCTTTTTAACGAAAGAGAACGATCAGAGCGC
CTCCTACTCGGCCCGCAATGTCATTCATGATCAGGAGAAGAAAAAACGAGGATTCGGATGGTTCAGACCGTTGCTTGGCG
GAGTGATCGGCGGCAGTCTTGCTCTTGGCATTTACACGTTTACACCGCTTGGTGACCATGATTCTCAGGACACTGCAAAA
CAATCATCCAGCCAGCAGCAAACGCAATCTGTTACAGCAACAAGCACCTCCTCTGAATCTAAAAAAAGCTCAAGCAGCTC
ATCTGCATTCAAGAGCGAGGACTCTTCTAAAATCTCAGATATGGTAGAAGACCTTTCACCAGCGATTGTCGGTATTACAA
ATCTTCAGGCACAATCAAACAGCTCTTTGTTCGGCTCTAGTTCTTCTGATTCCAGCGAAGATACAGAAAGCGGTTCAGGG
TCAGGTGTCATTTTCAAAAAAGAGAATGGCAAGGCTTATATCATTACAAATAACCACGTCGTAGAAGGGGCATCATCACT
GAAGGTATCTTTATATGACGGCACTGAGGTTACTGCAAAGCTGGTAGGCAGTGACTCGCTAACTGATTTAGCCGTCCTCC
AAATCAGTGATGACCACGTCACAAAAGTGGCAAACTTCGGTGATTCATCTGATCTTAGAACAGGCGAGACCGTTATTGCG
ATTGGGGATCCGCTTGGAAAAGACCTGTCCCGCACAGTAACACAAGGAATTGTAAGCGGCGTGGACAGAACGGTTTCAAT
GTCTACATCAGCCGGCGAAACGAGCATTAACGTCATTCAGACAGACGCAGCAATTAATCCAGGTAACAGCGGCGGTCCTT
TGTTAAATACAGACGGCAAAATCGTCGGCATTAACAGTATGAAAATCAGTGAAGATGATGTTGAGGGTATCGGATTTGCC
ATTCCAAGCAATGACGTAAAACCGATTGCTGAAGAATTACTGTCTAAAGGACAAATTGAACGTCCATATATCGGTGTCAG
CATGCTTGATCTAGAACAAGTGCCGCAAAATTACCAAGAAGGCACACTCGGCCTGTTCGGCAGCCAGCTGAATAAAGGTG
TTTACATCCGTGAGGTCGCTTCAGGCTCTCCTGCTGAAAAGGCCGGATTAAAAGCGGAGGATATTATCATCGGCCTAAAA
GGTAAAGAAATTGATACAGGCAGTGAATTGCGCAATATCTTATATAAAGACGCAAAGATCGGTGATACCGTTGAAGTGAA
AATTCTCCGAAACGGCAAAGAAATGACGAAAAAAATTAAACTGGATCAAAAAGAAGAGAAAACTTCGTAA

Domains

Predicted by InterProScan.

PDZ domain (PDZ_2)

(342-441)

Trypsin-like peptidase domain (Trypsin_2)

(160-304)

Peptidase family M50 (Peptidase_M50)

(366-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34358

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus pneumoniae Rx1

41.045

89.532

0.367
  htrA Streptococcus pneumoniae D39

41.045

89.532

0.367
  htrA Streptococcus pneumoniae R6

41.045

89.532

0.367
  htrA Streptococcus pneumoniae TIGR4

41.045

89.532

0.367
  htrA Streptococcus mitis NCTC 12261

39.563

91.759

0.363