Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   KIK02_RS17455 Genome accession   NZ_CP075171
Coordinates   3749532..3751529 (-) Length   665 a.a.
NCBI ID   WP_233743849.1    Uniprot ID   -
Organism   Leptodesmis sichuanensis A121     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3744532..3756529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIK02_RS17440 (KIK02_17580) - 3746270..3746878 (-) 609 WP_233743846.1 hypothetical protein -
  KIK02_RS17445 (KIK02_17585) - 3746897..3748123 (-) 1227 WP_233743847.1 type II secretion system F family protein -
  KIK02_RS17450 (KIK02_17590) pilT 3748237..3749352 (-) 1116 WP_233743848.1 type IV pilus twitching motility protein PilT Machinery gene
  KIK02_RS17455 (KIK02_17595) pilB/pilB1 3749532..3751529 (-) 1998 WP_233743849.1 GspE/PulE family protein Machinery gene
  KIK02_RS17460 (KIK02_17600) grpE 3751998..3752741 (+) 744 WP_233743850.1 nucleotide exchange factor GrpE -
  KIK02_RS17465 (KIK02_17605) dnaK 3752890..3754842 (+) 1953 WP_233743851.1 molecular chaperone DnaK -
  KIK02_RS17470 (KIK02_17610) dnaJ 3755052..3756185 (+) 1134 WP_233743852.1 molecular chaperone DnaJ -
  KIK02_RS17475 (KIK02_17615) - 3756182..3756460 (+) 279 WP_233743853.1 sulfurtransferase TusA family protein -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 74390.15 Da        Isoelectric Point: 4.9093

>NTDB_id=568210 KIK02_RS17455 WP_233743849.1 3749532..3751529(-) (pilB/pilB1) [Leptodesmis sichuanensis A121]
MTNSSSQRRALVVQNNFSPFGNKLVQSGYVDSDQMQQALIESRKTGRPLLDVLESLTGQQLPPDLIRQYKKQQLFELKIL
YGVESLDPEVSQIPTTQVGELIDTLISIDLCRRYRLVPLLRNDEEPRSVLVAMVDPDNLGAQDDLNRILRPQGLSLQRLV
ITLEDYQRLISSYLDQQVEKQKQIEAQQKVDIQADLEGLGYMDLQEAPEEDDADLDVADAEAAPVIALANKIMIKALQEG
VSDIHIEPQEEYLRVRFRKDGVLREAFEPFPKKIIPAVTARFKIMSQLDIAERRLPQDGRIRRRYDNRNIDFRVSTLPSK
YGEKVVLRILDNSATQLGLDKLITDPETLRIVQEMIARPFGLILVTGPTGSGKTTTLYSALAERNDPGVNISTAEDPIEY
TLPGLTQVQVIREKGMDFAMILRAFLRQDPDVILVGETRDRETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGVEP
FMVSGALIGVVAQRLVRRVCDECRIPYTPSRDELARFGLTTSGDGDITLYKANTLQSHEIQEAKAKNQLCPKCSGVGYKG
RCGVYEVMRVTERLQTLINQGAPTGTIKEAAVEEGMKTLLAYSLNLVREGLSTLEEVERVTFTDSGLEAELKAKRKSSLT
CRCCAAELKQEWLDCPYCLTARFHD

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=568210 KIK02_RS17455 WP_233743849.1 3749532..3751529(-) (pilB/pilB1) [Leptodesmis sichuanensis A121]
ATGACCAACTCCTCATCACAACGTCGTGCGCTTGTCGTACAAAATAACTTTTCTCCATTTGGAAATAAGCTAGTTCAGTC
TGGCTATGTTGACAGTGACCAGATGCAGCAGGCTCTAATTGAGAGCCGGAAAACGGGTAGACCGCTACTGGATGTTCTGG
AATCCCTAACTGGGCAGCAACTGCCACCGGATTTAATCAGACAGTACAAAAAGCAACAGCTATTTGAACTCAAGATTTTA
TATGGGGTAGAGTCACTTGACCCTGAGGTCAGCCAGATTCCCACAACTCAAGTTGGAGAACTGATTGATACCTTAATTTC
TATTGACTTGTGCCGTCGCTATCGATTAGTCCCGCTGCTTCGCAATGACGAAGAGCCACGATCGGTTCTGGTGGCGATGG
TAGATCCAGACAACTTGGGTGCTCAGGATGACCTGAATCGCATTTTGCGCCCTCAGGGGCTCAGCTTACAGCGGTTGGTG
ATCACATTAGAAGACTACCAGCGCCTGATTTCCAGCTATCTTGATCAGCAAGTTGAGAAGCAGAAGCAAATTGAAGCCCA
ACAGAAGGTTGACATTCAGGCCGATCTGGAAGGGCTGGGCTACATGGATCTTCAGGAGGCCCCTGAAGAAGACGATGCCG
ATCTAGATGTAGCCGATGCCGAGGCTGCTCCGGTTATCGCTCTGGCTAACAAGATTATGATCAAGGCACTCCAGGAGGGC
GTGTCTGACATTCACATTGAGCCACAGGAAGAATATTTGCGAGTCCGGTTCCGGAAAGACGGGGTATTGCGAGAAGCCTT
TGAGCCATTTCCCAAGAAGATTATTCCAGCGGTAACGGCTCGCTTCAAGATCATGTCTCAACTAGATATTGCTGAACGAC
GGCTGCCGCAAGATGGCCGGATCCGACGACGCTATGACAATCGCAATATTGACTTCCGGGTTAGTACATTGCCCAGTAAG
TATGGCGAAAAGGTCGTATTGCGGATTCTGGATAATTCAGCCACCCAATTAGGCTTGGACAAGCTGATTACGGATCCAGA
AACCCTCCGCATCGTGCAAGAGATGATTGCTCGTCCTTTTGGTCTGATTCTGGTTACCGGGCCAACGGGTTCCGGAAAAA
CGACCACTCTCTATTCGGCTTTAGCAGAACGGAATGATCCCGGTGTCAATATCAGTACGGCAGAAGACCCTATTGAATAT
ACCCTGCCCGGTTTAACCCAGGTGCAGGTGATTCGGGAGAAGGGGATGGACTTCGCTATGATTCTGCGAGCCTTCCTGCG
CCAAGACCCGGATGTCATCCTGGTGGGTGAGACGCGAGACCGGGAAACGGCCAAGACGGCGATCGAAGCCGCTCTCACTG
GACACCTGGTATTAACAACTCTGCACACCAATGACGCGGCAGGGGCGATCGCACGTCTGGATGAAATGGGGGTGGAACCC
TTCATGGTTTCTGGGGCACTGATTGGAGTCGTGGCCCAACGTCTGGTACGCCGTGTCTGTGACGAATGCCGCATTCCCTA
CACCCCTAGCCGGGATGAACTGGCCCGATTTGGGTTAACAACCTCTGGGGATGGAGATATTACGCTATACAAAGCCAATA
CCTTACAATCCCACGAAATTCAGGAGGCCAAAGCTAAAAATCAGCTTTGTCCAAAGTGTAGTGGAGTAGGGTACAAGGGA
CGGTGCGGTGTCTATGAAGTGATGCGGGTGACGGAGCGCTTACAAACGTTGATTAATCAGGGAGCACCCACAGGCACGAT
CAAAGAAGCTGCGGTCGAAGAAGGGATGAAAACCCTGCTGGCTTACAGCTTAAATCTGGTACGTGAGGGGTTAAGCACTC
TGGAGGAAGTGGAACGGGTGACTTTTACAGATTCGGGCTTAGAAGCCGAACTGAAAGCCAAGCGCAAGAGTTCTTTAACA
TGCCGTTGTTGTGCCGCTGAACTCAAGCAAGAATGGCTGGATTGTCCGTACTGTCTCACGGCCCGTTTCCATGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-178)

Type II/IV secretion system protein (T2SSE)

(231-498)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

67.927

99.398

0.675
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.903

93.083

0.371
  pilF Thermus thermophilus HB27

39.286

92.632

0.364