Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MJ614_RS10715 Genome accession   NZ_CP092647
Coordinates   1748166..1748825 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain EC5502     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1743166..1753825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ614_RS10695 plsC 1743312..1744049 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  MJ614_RS10700 parC 1744283..1746541 (-) 2259 WP_001281840.1 DNA topoisomerase IV subunit A -
  MJ614_RS10705 ygiV 1747087..1747569 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  MJ614_RS10710 ygiW 1747622..1748014 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  MJ614_RS10715 ciaR 1748166..1748825 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  MJ614_RS10720 qseC 1748822..1750171 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  MJ614_RS10725 ygiZ 1750217..1750549 (-) 333 WP_000912120.1 DUF2645 family protein -
  MJ614_RS10730 mdaB 1750868..1751449 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  MJ614_RS10735 ygiN 1751480..1751794 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  MJ614_RS10740 parE 1751842..1753734 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=567692 MJ614_RS10715 WP_001221502.1 1748166..1748825(+) (ciaR) [Escherichia coli strain EC5502]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=567692 MJ614_RS10715 WP_001221502.1 1748166..1748825(+) (ciaR) [Escherichia coli strain EC5502]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365