Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KH277_RS07685 Genome accession   NZ_CP075054
Coordinates   1490654..1491724 (+) Length   356 a.a.
NCBI ID   WP_213390994.1    Uniprot ID   -
Organism   Bacillus velezensis strain VTX20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1485654..1496724
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH277_RS07655 (KH277_07655) - 1486129..1486374 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  KH277_RS07660 (KH277_07660) - 1486611..1486991 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KH277_RS07670 (KH277_07670) - 1487197..1488027 (+) 831 WP_003153080.1 STAS domain-containing protein -
  KH277_RS07675 (KH277_07675) - 1488065..1489366 (-) 1302 WP_014305416.1 hemolysin family protein -
  KH277_RS07680 (KH277_07680) - 1489512..1490462 (+) 951 WP_003153082.1 magnesium transporter CorA family protein -
  KH277_RS07685 (KH277_07685) comGA 1490654..1491724 (+) 1071 WP_213390994.1 competence type IV pilus ATPase ComGA Machinery gene
  KH277_RS07690 (KH277_07690) comGB 1491711..1492748 (+) 1038 WP_213390995.1 competence type IV pilus assembly protein ComGB Machinery gene
  KH277_RS07695 (KH277_07695) comGC 1492795..1493061 (+) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  KH277_RS07700 (KH277_07700) comGD 1493051..1493488 (+) 438 WP_014305413.1 competence type IV pilus minor pilin ComGD Machinery gene
  KH277_RS07705 (KH277_07705) comGE 1493472..1493786 (+) 315 WP_003153089.1 competence type IV pilus minor pilin ComGE -
  KH277_RS07710 (KH277_07710) comGF 1493800..1494195 (+) 396 WP_003153090.1 competence type IV pilus minor pilin ComGF -
  KH277_RS07715 (KH277_07715) comGG 1494196..1494573 (+) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  KH277_RS07720 (KH277_07720) - 1494630..1494809 (+) 180 WP_003153093.1 YqzE family protein -
  KH277_RS07725 (KH277_07725) - 1494849..1495178 (-) 330 WP_003153097.1 DUF3889 domain-containing protein -
  KH277_RS07730 (KH277_07730) tapA 1495437..1496108 (+) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  KH277_RS07735 (KH277_07735) - 1496080..1496664 (+) 585 WP_012117977.1 signal peptidase I -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40519.81 Da        Isoelectric Point: 9.4729

>NTDB_id=567468 KH277_RS07685 WP_213390994.1 1490654..1491724(+) (comGA) [Bacillus velezensis strain VTX20]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPSLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=567468 KH277_RS07685 WP_213390994.1 1490654..1491724(+) (comGA) [Bacillus velezensis strain VTX20]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCTCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGTTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATAGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.506

97.753

0.376
  pilF Thermus thermophilus HB27

37.892

98.596

0.374