Detailed information NTG0567464

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: KH277_RS07260
• Genome accession: NZ_CP075054
• Coordinates: 1414603..1415217 (+)
• Length: 204 a.a.
• NCBI ID: WP_015387983.1
• Uniprot ID: A0A6A8LHQ3
• Organism: Bacillus velezensis strain VTX20
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1409603..1420217

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KH277_RS07225 (KH277_07225)	aroE	1410290..1411132 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
KH277_RS07230 (KH277_07230)	yhbY	1411126..1411416 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KH277_RS07235 (KH277_07235)	-	1411427..1411996 (+)	570	WP_014305453.1	nicotinate-nucleotide adenylyltransferase	-
KH277_RS07240 (KH277_07240)	yqeK	1411986..1412567 (+)	582	WP_014305452.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KH277_RS07245 (KH277_07245)	rsfS	1412564..1412920 (+)	357	WP_003152864.1	ribosome silencing factor	-
KH277_RS07250 (KH277_07250)	-	1412917..1413654 (+)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
KH277_RS07255 (KH277_07255)	comER	1413723..1414544 (-)	822	WP_003152866.1	late competence protein ComER	-
KH277_RS07260 (KH277_07260)	comEA	1414603..1415217 (+)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
KH277_RS07265 (KH277_07265)	-	1415284..1415853 (+)	570	WP_003152868.1	ComE operon protein 2	-
KH277_RS07270 (KH277_07270)	comEC	1415854..1418205 (+)	2352	WP_213390988.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KH277_RS07275 (KH277_07275)	-	1418224..1418358 (-)	135	WP_213390989.1	YqzM family protein	-
KH277_RS07280 (KH277_07280)	-	1418399..1418553 (+)	155	Protein_1420	hypothetical protein	-
KH277_RS07285 (KH277_07285)	holA	1418593..1419634 (+)	1042	Protein_1421	DNA polymerase III subunit delta	-
KH277_RS07290 (KH277_07290)	rpsT	1419651..1419917 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22255.41 Da
• Isoelectric Point: 5.5211

>NTDB_id=567464 KH277_RS07260 WP_015387983.1 1414603..1415217(+) (comEA) [Bacillus velezensis strain VTX20]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=567464 KH277_RS07260 WP_015387983.1 1414603..1415217(+) (comEA) [Bacillus velezensis strain VTX20]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A6A8LHQ3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363