Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KH263_RS12385 Genome accession   NZ_CP074685
Coordinates   2593380..2593994 (-) Length   204 a.a.
NCBI ID   WP_213403489.1    Uniprot ID   -
Organism   Bacillus velezensis strain VTX9     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2588380..2598994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KH263_RS12355 (KH263_12355) rpsT 2588680..2588946 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  KH263_RS12360 (KH263_12360) holA 2588963..2590004 (-) 1042 Protein_2409 DNA polymerase III subunit delta -
  KH263_RS12365 (KH263_12365) - 2590044..2590198 (-) 155 Protein_2410 hypothetical protein -
  KH263_RS12370 (KH263_12370) - 2590239..2590373 (+) 135 WP_003152870.1 YqzM family protein -
  KH263_RS12375 (KH263_12375) comEC 2590392..2592743 (-) 2352 WP_213403488.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KH263_RS12380 (KH263_12380) - 2592744..2593313 (-) 570 WP_003152868.1 ComE operon protein 2 -
  KH263_RS12385 (KH263_12385) comEA 2593380..2593994 (-) 615 WP_213403489.1 helix-hairpin-helix domain-containing protein Machinery gene
  KH263_RS12390 (KH263_12390) comER 2594053..2594874 (+) 822 WP_014305449.1 late competence protein ComER -
  KH263_RS12395 (KH263_12395) - 2594943..2595680 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  KH263_RS12400 (KH263_12400) rsfS 2595677..2596033 (-) 357 WP_003152864.1 ribosome silencing factor -
  KH263_RS12405 (KH263_12405) yqeK 2596030..2596611 (-) 582 WP_003152863.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KH263_RS12410 (KH263_12410) - 2596601..2597170 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  KH263_RS12415 (KH263_12415) yhbY 2597181..2597471 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  KH263_RS12420 (KH263_12420) aroE 2597465..2598307 (-) 843 WP_025852995.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22164.34 Da        Isoelectric Point: 6.9685

>NTDB_id=565739 KH263_RS12385 WP_213403489.1 2593380..2593994(-) (comEA) [Bacillus velezensis strain VTX9]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGGVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEGNHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMKVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=565739 KH263_RS12385 WP_213403489.1 2593380..2593994(-) (comEA) [Bacillus velezensis strain VTX9]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGGGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGGGAATCATCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAAGGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.333

94.118

0.549
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363