Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KIH94_RS12165 Genome accession   NZ_CP074571
Coordinates   2380656..2381726 (-) Length   356 a.a.
NCBI ID   WP_015483431.1    Uniprot ID   -
Organism   Bacillus subtilis strain BYS2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2375656..2386726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIH94_RS12120 (KIH94_12120) tapA 2376136..2376897 (-) 762 WP_015384085.1 amyloid fiber anchoring/assembly protein TapA -
  KIH94_RS12125 (KIH94_12125) yqzG 2377167..2377493 (+) 327 WP_015384086.1 YqzG/YhdC family protein -
  KIH94_RS12130 (KIH94_12130) spoIITA 2377535..2377714 (-) 180 WP_003230176.1 YqzE family protein -
  KIH94_RS12135 (KIH94_12135) comGG 2377786..2378160 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  KIH94_RS12140 (KIH94_12140) comGF 2378161..2378544 (-) 384 WP_015384087.1 ComG operon protein ComGF Machinery gene
  KIH94_RS12145 (KIH94_12145) comGE 2378570..2378917 (-) 348 WP_015384088.1 ComG operon protein 5 Machinery gene
  KIH94_RS12150 (KIH94_12150) comGD 2378901..2379332 (-) 432 WP_015384089.1 comG operon protein ComGD Machinery gene
  KIH94_RS12155 (KIH94_12155) comGC 2379322..2379618 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  KIH94_RS12160 (KIH94_12160) comGB 2379632..2380669 (-) 1038 WP_015483430.1 comG operon protein ComGB Machinery gene
  KIH94_RS12165 (KIH94_12165) comGA 2380656..2381726 (-) 1071 WP_015483431.1 competence protein ComGA Machinery gene
  KIH94_RS12170 (KIH94_12170) corA 2382136..2383089 (-) 954 WP_015483432.1 magnesium transporter CorA -
  KIH94_RS12175 (KIH94_12175) yqhB 2383232..2384560 (+) 1329 WP_003230150.1 hemolysin family protein -
  KIH94_RS12180 (KIH94_12180) rsbRD 2384613..2385449 (-) 837 WP_015384093.1 RsbT co-antagonist protein RsbRD -
  KIH94_RS12190 (KIH94_12190) mgsR 2385673..2386053 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  KIH94_RS12195 (KIH94_12195) yqgY 2386285..2386530 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=565314 KIH94_RS12165 WP_015483431.1 2380656..2381726(-) (comGA) [Bacillus subtilis strain BYS2]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYITTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=565314 KIH94_RS12165 WP_015483431.1 2380656..2381726(-) (comGA) [Bacillus subtilis strain BYS2]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGTGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTAGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATATAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997
  pilF Thermus thermophilus HB27

36.827

99.157

0.365