Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J5O00_RS15185 Genome accession   NZ_CP074415
Coordinates   3184417..3185010 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain 64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3179417..3190010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5O00_RS15165 aroB 3180293..3181393 (-) 1101 WP_005496576.1 3-dehydroquinate synthase -
  J5O00_RS15170 aroK 3181425..3181943 (-) 519 WP_005381319.1 shikimate kinase AroK -
  J5O00_RS15175 pilQ 3182132..3183880 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  J5O00_RS15180 pilP 3183912..3184427 (-) 516 WP_017449464.1 pilus assembly protein PilP Machinery gene
  J5O00_RS15185 pilO 3184417..3185010 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  J5O00_RS15190 pilN 3185003..3185581 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  J5O00_RS15195 pilM 3185565..3186581 (-) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  J5O00_RS15200 - 3186724..3189294 (+) 2571 WP_029804285.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=564795 J5O00_RS15185 WP_005480482.1 3184417..3185010(-) (pilO) [Vibrio parahaemolyticus strain 64]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=564795 J5O00_RS15185 WP_005480482.1 3184417..3185010(-) (pilO) [Vibrio parahaemolyticus strain 64]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726