Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   J5O00_RS15195 Genome accession   NZ_CP074415
Coordinates   3185565..3186581 (-) Length   338 a.a.
NCBI ID   WP_005496568.1    Uniprot ID   A0A3E1IL27
Organism   Vibrio parahaemolyticus strain 64     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3180565..3191581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5O00_RS15170 aroK 3181425..3181943 (-) 519 WP_005381319.1 shikimate kinase AroK -
  J5O00_RS15175 pilQ 3182132..3183880 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  J5O00_RS15180 pilP 3183912..3184427 (-) 516 WP_017449464.1 pilus assembly protein PilP Machinery gene
  J5O00_RS15185 pilO 3184417..3185010 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  J5O00_RS15190 pilN 3185003..3185581 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  J5O00_RS15195 pilM 3185565..3186581 (-) 1017 WP_005496568.1 type IV pilus assembly protein PilM Machinery gene
  J5O00_RS15200 - 3186724..3189294 (+) 2571 WP_029804285.1 PBP1A family penicillin-binding protein -
  J5O00_RS15205 oxyR 3189350..3190258 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  J5O00_RS15210 - 3190429..3191157 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8323

>NTDB_id=564797 J5O00_RS15195 WP_005496568.1 3185565..3186581(-) (pilM) [Vibrio parahaemolyticus strain 64]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=564797 J5O00_RS15195 WP_005496568.1 3185565..3186581(-) (pilM) [Vibrio parahaemolyticus strain 64]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCAACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGGTGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1IL27

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

85.207

100

0.852
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482