Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KHA81_RS12440 Genome accession   NZ_CP074391
Coordinates   2571251..2571865 (-) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus amyloliquefaciens strain L-17     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2566251..2576865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA81_RS12410 rpsT 2566551..2566817 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  KHA81_RS12415 holA 2566834..2567875 (-) 1042 Protein_2414 DNA polymerase III subunit delta -
  KHA81_RS12420 - 2567915..2568069 (-) 155 Protein_2415 hypothetical protein -
  KHA81_RS12425 - 2568110..2568244 (+) 135 WP_003152870.1 YqzM family protein -
  KHA81_RS12430 comEC 2568263..2570614 (-) 2352 WP_015387984.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KHA81_RS12435 - 2570615..2571184 (-) 570 WP_003152868.1 ComE operon protein 2 -
  KHA81_RS12440 comEA 2571251..2571865 (-) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  KHA81_RS12445 comER 2571924..2572745 (+) 822 WP_003152866.1 late competence protein ComER -
  KHA81_RS12450 - 2572814..2573551 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  KHA81_RS12455 rsfS 2573548..2573904 (-) 357 WP_014305451.1 ribosome silencing factor -
  KHA81_RS12460 yqeK 2573901..2574482 (-) 582 WP_015387982.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KHA81_RS12465 - 2574472..2575041 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  KHA81_RS12470 yhbY 2575052..2575342 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  KHA81_RS12475 aroE 2575336..2576178 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=564569 KHA81_RS12440 WP_015387983.1 2571251..2571865(-) (comEA) [Bacillus amyloliquefaciens strain L-17]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=564569 KHA81_RS12440 WP_015387983.1 2571251..2571865(-) (comEA) [Bacillus amyloliquefaciens strain L-17]
ATGACGAAAGCAATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363