Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KHA81_RS12015 Genome accession   NZ_CP074391
Coordinates   2494739..2495809 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain L-17     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2489739..2500809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA81_RS11965 sipW 2489798..2490382 (-) 585 WP_012117977.1 signal peptidase I SipW -
  KHA81_RS11970 tapA 2490354..2491025 (-) 672 WP_015388007.1 amyloid fiber anchoring/assembly protein TapA -
  KHA81_RS11975 - 2491285..2491614 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  KHA81_RS11980 - 2491654..2491833 (-) 180 WP_003153093.1 YqzE family protein -
  KHA81_RS11985 comGG 2491890..2492267 (-) 378 WP_015388005.1 competence type IV pilus minor pilin ComGG Machinery gene
  KHA81_RS11990 comGF 2492268..2492768 (-) 501 WP_225879914.1 competence type IV pilus minor pilin ComGF -
  KHA81_RS11995 comGE 2492677..2492991 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  KHA81_RS12000 comGD 2492975..2493412 (-) 438 WP_015388002.1 competence type IV pilus minor pilin ComGD Machinery gene
  KHA81_RS12005 comGC 2493402..2493668 (-) 267 WP_042635730.1 competence type IV pilus major pilin ComGC Machinery gene
  KHA81_RS12010 comGB 2493715..2494752 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  KHA81_RS12015 comGA 2494739..2495809 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  KHA81_RS12020 - 2496001..2496951 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  KHA81_RS12025 - 2497097..2498398 (+) 1302 WP_014305416.1 hemolysin family protein -
  KHA81_RS12030 - 2498437..2499267 (-) 831 WP_003153080.1 STAS domain-containing protein -
  KHA81_RS12040 - 2499473..2499853 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  KHA81_RS12045 - 2500090..2500335 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=564565 KHA81_RS12015 WP_003153083.1 2494739..2495809(-) (comGA) [Bacillus amyloliquefaciens strain L-17]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=564565 KHA81_RS12015 WP_003153083.1 2494739..2495809(-) (comGA) [Bacillus amyloliquefaciens strain L-17]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374