Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   KGD89_RS21690 Genome accession   NZ_CP074349
Coordinates   5103910..5105250 (+) Length   446 a.a.
NCBI ID   WP_025261861.1    Uniprot ID   A0A3M4W6G4
Organism   Pseudomonas cichorii strain DSM 50259     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5098910..5110250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGD89_RS21665 (KGD89_21660) pgeF 5099030..5099758 (-) 729 WP_025261856.1 peptidoglycan editing factor PgeF -
  KGD89_RS21670 (KGD89_21665) rluD 5099755..5100717 (-) 963 WP_025261857.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  KGD89_RS21675 (KGD89_21670) - 5100867..5101889 (+) 1023 WP_025261858.1 outer membrane protein assembly factor BamD -
  KGD89_RS21680 (KGD89_21675) - 5102086..5102325 (+) 240 WP_038400034.1 PP0621 family protein -
  KGD89_RS21685 (KGD89_21680) pilS 5102315..5103907 (+) 1593 WP_025261860.1 PAS domain-containing sensor histidine kinase Regulator
  KGD89_RS21690 (KGD89_21685) pilR 5103910..5105250 (+) 1341 WP_025261861.1 sigma-54 dependent transcriptional regulator Regulator
  KGD89_RS21695 (KGD89_21690) thiO 5105276..5106388 (-) 1113 WP_025261862.1 glycine oxidase ThiO -
  KGD89_RS21700 (KGD89_21695) - 5106417..5106815 (-) 399 WP_038400035.1 type IV pilin protein -
  KGD89_RS21705 (KGD89_21700) - 5106828..5110010 (-) 3183 WP_025261864.1 pilus assembly protein -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 50223.48 Da        Isoelectric Point: 6.6404

>NTDB_id=564129 KGD89_RS21690 WP_025261861.1 5103910..5105250(+) (pilR) [Pseudomonas cichorii strain DSM 50259]
MTQRQKILIVDDEPDIRELLEITLGRMKLDTHSARNVTEAREWLAREPFDMCLTDMRLPDGNGLELVQHIQQVYPQTPVA
MITAHGSLDMAIHALKAGAFDFLTKPVDLTRLRELVSSALRLKPGAPAERSIDSRLLGNSPPMRALRKQIDKLARSQAPI
YISGESGSGKELVARLIHEQGPRIDKPFVPVNCGAIPSELMESEFFGHRKGSFTGAHEDKPGLFQAANNGTLFLDEVADL
PLAMQVKLLRAIQEKSIRSVGGQQEQVVDVRILCATHKDLNAEVAAGRFRQDLYYRLNVIELRVPPLRERREDIDQLVGS
VLLRLAANCGQASARLHPQALETLKSYRFPGNVRELENMLERAYTLCENDEIHASDLRLAESGTPQEHDGHNLADIDNLE
DYLESIERKLILQALEETRWNRTAAAQRLSLSFRSMRYRLKKLGLD

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=564129 KGD89_RS21690 WP_025261861.1 5103910..5105250(+) (pilR) [Pseudomonas cichorii strain DSM 50259]
ATGACCCAACGGCAAAAGATCCTGATAGTCGATGATGAGCCGGACATTCGTGAACTCCTGGAGATCACCCTCGGCCGCAT
GAAACTCGATACCCACAGCGCCCGCAATGTGACCGAGGCCCGCGAGTGGCTGGCCCGCGAGCCATTCGACATGTGCCTGA
CCGACATGCGCCTGCCTGACGGCAATGGCCTGGAGCTGGTGCAGCATATTCAGCAGGTTTACCCACAGACACCCGTGGCA
ATGATCACCGCCCATGGCAGCCTGGATATGGCCATCCATGCACTGAAGGCCGGGGCATTCGACTTCCTGACCAAACCGGT
GGACCTGACCCGGCTTCGTGAACTGGTCAGCAGCGCCTTGCGTCTGAAGCCCGGCGCCCCGGCAGAACGCAGCATCGACA
GTCGTCTGCTGGGCAACTCCCCACCGATGCGTGCCTTGCGCAAGCAAATCGACAAACTGGCTCGCAGCCAGGCGCCGATC
TATATCAGTGGCGAATCGGGGAGCGGCAAGGAACTGGTCGCGCGGCTGATTCATGAGCAAGGGCCACGCATCGACAAGCC
TTTCGTGCCAGTCAACTGCGGGGCGATACCGTCGGAATTGATGGAAAGCGAGTTCTTCGGCCATCGCAAAGGCAGCTTTA
CCGGCGCCCATGAAGACAAGCCGGGGCTGTTTCAGGCGGCCAATAACGGCACGCTGTTTCTGGATGAAGTCGCTGACTTG
CCGCTGGCCATGCAGGTAAAGCTACTGCGGGCCATACAGGAAAAATCCATCCGCAGCGTCGGTGGTCAGCAAGAGCAAGT
GGTGGATGTGCGCATTCTCTGCGCCACCCACAAGGACTTGAACGCCGAGGTCGCTGCCGGGCGCTTCCGGCAGGATTTGT
ATTACCGCCTGAACGTCATCGAACTGCGAGTGCCGCCCTTGCGTGAGCGGCGTGAAGATATCGACCAACTGGTCGGCAGT
GTCCTGCTGCGTCTGGCGGCCAACTGCGGCCAGGCCTCGGCACGCCTGCACCCTCAGGCGCTGGAAACCCTGAAAAGCTA
CCGCTTCCCCGGCAATGTCCGCGAGCTGGAAAACATGCTGGAGCGTGCTTACACCCTTTGCGAAAACGATGAAATCCACG
CCTCGGACCTTCGCCTGGCCGAGTCCGGTACGCCTCAGGAGCACGACGGCCACAATCTGGCCGATATCGATAACCTGGAA
GATTACCTGGAAAGCATCGAACGCAAACTGATCCTGCAAGCCCTGGAGGAAACCCGCTGGAACCGGACTGCGGCAGCGCA
GCGCCTGAGCCTGTCATTCAGATCGATGCGTTACCGGTTGAAGAAGCTGGGGCTGGATTGA

Domains

Predicted by InterproScan.

Bacterial regulatory protein, Fis family (HTH_8)

(403-443)

Response regulator receiver domain (Response_reg)

(7-116)

Sigma-54 interaction domain (Sigma54_activat)

(136-302)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M4W6G4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

80.135

99.327

0.796
  pilR Acinetobacter baumannii strain A118

48.276

100

0.502