Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   KGD89_RS21685 Genome accession   NZ_CP074349
Coordinates   5102315..5103907 (+) Length   530 a.a.
NCBI ID   WP_025261860.1    Uniprot ID   A0A3M4W8Q9
Organism   Pseudomonas cichorii strain DSM 50259     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5097315..5108907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGD89_RS21665 (KGD89_21660) pgeF 5099030..5099758 (-) 729 WP_025261856.1 peptidoglycan editing factor PgeF -
  KGD89_RS21670 (KGD89_21665) rluD 5099755..5100717 (-) 963 WP_025261857.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  KGD89_RS21675 (KGD89_21670) - 5100867..5101889 (+) 1023 WP_025261858.1 outer membrane protein assembly factor BamD -
  KGD89_RS21680 (KGD89_21675) - 5102086..5102325 (+) 240 WP_038400034.1 PP0621 family protein -
  KGD89_RS21685 (KGD89_21680) pilS 5102315..5103907 (+) 1593 WP_025261860.1 PAS domain-containing sensor histidine kinase Regulator
  KGD89_RS21690 (KGD89_21685) pilR 5103910..5105250 (+) 1341 WP_025261861.1 sigma-54 dependent transcriptional regulator Regulator
  KGD89_RS21695 (KGD89_21690) thiO 5105276..5106388 (-) 1113 WP_025261862.1 glycine oxidase ThiO -
  KGD89_RS21700 (KGD89_21695) - 5106417..5106815 (-) 399 WP_038400035.1 type IV pilin protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58514.49 Da        Isoelectric Point: 6.4496

>NTDB_id=564128 KGD89_RS21685 WP_025261860.1 5102315..5103907(+) (pilS) [Pseudomonas cichorii strain DSM 50259]
MTAETLSLGSPQAQRILRLYHLYRLTIGIVLVLLISSDLDTELLQLANLNLFRAGCWLYLVFNILVVVLLERPSHKVQLF
SLAVTDTVMLSGLFYAAGGTSSGIGNLLIASVAISNVLLRGRLGLLIAAVSAIGIIYLTFFISFISPLASNDYVQASTLG
ALCFAAAMLVQALTARMQVSEVLAEQRAADVDQLEELNGLILQRMRTGILVLDSRRQVLLANQGALSLLGHEQLVGQVID
QYSPQLVDRLRQWLINPIMAPRSISTSAIGPILQPSFAALNRGDQRQTLIFLEDLSQVAHQAQQLKLAALGRLTAGIAHE
IRNPLGAVSHAAQLLNESEELSAPDRRLGQIIHDQSRRINVVIENVLQLSRRRETEPELLDLRTWLESFVADFRSSAPAN
QNVHTEIGQGMLTTRMDPEQLNQVLTNLLHNGLRYSGKNHDIAQVWLKLFHNPENDLPTLEVLDDGPGVSEQHLAKLFEP
FFTTESKGTGLGLYLSRELCESNQAHLDYSLRHEGGSCMRITFAHPFKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=564128 KGD89_RS21685 WP_025261860.1 5102315..5103907(+) (pilS) [Pseudomonas cichorii strain DSM 50259]
GTGACCGCTGAGACGCTTTCACTCGGCAGCCCCCAAGCGCAACGGATCCTGCGGCTGTATCACCTTTATCGACTGACCAT
TGGCATCGTGCTGGTGCTGTTGATCTCCAGCGACCTGGACACCGAACTGTTGCAACTGGCCAACCTGAATCTGTTTCGGG
CTGGCTGCTGGCTGTATCTGGTATTCAACATTCTGGTTGTGGTGCTGCTTGAGCGCCCCAGCCATAAAGTACAGCTCTTC
AGCCTGGCAGTGACCGATACGGTGATGCTGTCAGGGCTGTTCTATGCAGCGGGCGGCACTTCAAGTGGTATCGGCAATCT
GCTGATTGCCTCGGTCGCCATCAGTAATGTCCTGTTGCGCGGCCGCCTTGGTCTGTTGATTGCTGCTGTTTCCGCCATCG
GCATCATTTACCTGACTTTCTTCATCAGCTTCATCAGCCCGCTGGCCTCAAATGATTATGTGCAGGCCAGTACGCTGGGC
GCCTTGTGCTTTGCCGCCGCCATGCTGGTACAGGCACTGACCGCCCGCATGCAGGTGAGCGAAGTACTGGCCGAACAGCG
TGCCGCCGATGTCGACCAGCTCGAAGAACTCAATGGCCTGATCCTGCAACGCATGCGCACCGGCATTCTGGTGCTCGACT
CGCGCCGCCAGGTGCTGCTGGCCAATCAGGGCGCCTTGAGCCTGCTGGGCCATGAGCAACTGGTCGGCCAGGTCATCGAT
CAATACTCGCCACAACTGGTGGATCGCCTCCGGCAATGGCTGATCAACCCCATCATGGCACCGCGCAGCATATCGACCTC
AGCCATCGGCCCGATCCTGCAACCGAGCTTTGCCGCACTCAACCGTGGCGACCAGCGCCAGACGCTGATCTTTCTGGAAG
ACCTCTCGCAGGTTGCCCATCAGGCCCAGCAACTGAAGCTTGCAGCATTGGGTCGTCTGACAGCCGGTATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCTGTCAGCCACGCGGCGCAACTGCTCAATGAATCGGAAGAGCTGTCAGCACCGGACCGACG
ACTCGGGCAGATCATTCATGATCAGTCGCGACGCATCAATGTGGTGATCGAGAACGTCCTGCAACTGTCCAGGCGCCGCG
AAACCGAACCCGAACTGCTTGACCTGCGAACCTGGCTGGAGAGTTTCGTCGCGGACTTTCGCAGTTCCGCACCAGCCAAC
CAAAATGTGCATACCGAGATCGGCCAGGGCATGCTCACCACGCGCATGGACCCGGAGCAATTGAATCAGGTGCTGACCAA
CCTGCTGCACAACGGCTTACGGTACAGCGGCAAGAACCATGACATAGCGCAGGTCTGGCTGAAACTGTTCCACAATCCGG
AAAACGACCTACCGACCCTGGAAGTTCTCGATGACGGCCCTGGCGTATCCGAGCAGCATCTGGCCAAACTCTTCGAGCCC
TTTTTCACCACAGAAAGCAAAGGTACCGGCCTTGGCCTCTACCTTTCCCGGGAACTGTGCGAAAGCAATCAGGCGCATCT
GGATTACTCCCTTCGCCACGAAGGCGGTAGCTGCATGCGTATCACTTTCGCTCACCCGTTCAAGTTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M4W8Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

62.264

100

0.623