Detailed information NTG0563474

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: L6V99_RS03570
• Genome accession: NZ_CP091773
• Coordinates: 737840..738499 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli strain SWYH.B349aT
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 732840..743499

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L6V99_RS03540 (L6V99_03540)	ygiN	733741..734055 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
L6V99_RS03545 (L6V99_03545)	mdaB	734086..734667 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
L6V99_RS03550 (L6V99_03550)	-	734917..735396 (+)	480	WP_000065332.1	Hcp family type VI secretion system effector	-
L6V99_RS03555 (L6V99_03555)	-	735399..736109 (+)	711	WP_000834030.1	hypothetical protein	-
L6V99_RS03560 (L6V99_03560)	ygiZ	736116..736448 (+)	333	WP_000914690.1	DUF2645 family protein	-
L6V99_RS03565 (L6V99_03565)	qseC	736494..737843 (-)	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
L6V99_RS03570 (L6V99_03570)	ciaR	737840..738499 (-)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
L6V99_RS03575 (L6V99_03575)	ygiW	738651..739043 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
L6V99_RS03580 (L6V99_03580)	ygiV	739096..739578 (+)	483	WP_000183505.1	AraC family transcriptional regulator	-
L6V99_RS03585 (L6V99_03585)	mqsR	739783..740079 (+)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
L6V99_RS03590 (L6V99_03590)	mqsA	740081..740476 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
L6V99_RS03595 (L6V99_03595)	ygiS	740609..742216 (+)	1608	WP_001375094.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=563474 L6V99_RS03570 WP_001221502.1 737840..738499(-) (ciaR) [Escherichia coli strain SWYH.B349aT]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=563474 L6V99_RS03570 WP_001221502.1 737840..738499(-) (ciaR) [Escherichia coli strain SWYH.B349aT]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365