Detailed information NTG0561785

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LVJ79_RS08635
• Genome accession: NZ_CP091518
• Coordinates: 1621455..1622735 (-)
• Length: 426 a.a.
• NCBI ID: WP_003787551.1
• Uniprot ID: F5S877
• Organism: Kingella kingae strain 4177/66
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1616455..1627735

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LVJ79_RS08610 (LVJ79_08610)	folD	1617476..1618327 (+)	852	WP_003787562.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD	-
LVJ79_RS08615 (LVJ79_08615)	-	1618470..1619855 (+)	1386	WP_003787560.1	GntP family permease	-
LVJ79_RS08620 (LVJ79_08620)	-	1619980..1620489 (+)	510	WP_003787558.1	gluconokinase	-
LVJ79_RS08625 (LVJ79_08625)	-	1620560..1620937 (-)	378	WP_003787555.1	diacylglycerol kinase	-
LVJ79_RS08630 (LVJ79_08630)	-	1620934..1621377 (-)	444	WP_003787553.1	peroxiredoxin	-
LVJ79_RS08635 (LVJ79_08635)	pilU	1621455..1622735 (-)	1281	WP_003787551.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LVJ79_RS08640 (LVJ79_08640)	pilT	1622755..1623795 (-)	1041	WP_003791105.1	type IV pilus twitching motility protein PilT	Machinery gene
LVJ79_RS08645 (LVJ79_08645)	pseC	1624001..1625158 (-)	1158	WP_003787545.1	UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase	-
LVJ79_RS08650 (LVJ79_08650)	-	1625271..1626446 (-)	1176	WP_003787543.1	alpha-hydroxy acid oxidase	-

Sequence

Protein

• Length: 426 a.a.
• Molecular weight: 47321.06 Da
• Isoelectric Point: 6.2389

>NTDB_id=561785 LVJ79_RS08635 WP_003787551.1 1621455..1622735(-) (pilU) [Kingella kingae strain 4177/66]
MAEQDITSLLEGFVNNYKVTNDAAKPAAPAGRAVPASAQATATASEIAPAKKSGLHPLLEKMAAECEKRGASDIFISAGF
PPSFKINGELTPVPLKPLTQQDTQILVYSTMSPDQRARFDHELELNYSVSSSEGIRFRVNSYHEQGRMGMVWRRITTKIL
TVDDLQLPQGLKDLAMQPRGLLILAGATGSGKSTSMAAMLDWRNKHAAGHIVTIEDPVEYLHKPIKSIFTQRELGVDTNS
WSMAVQSAMRQAPDVVCVGEVRNEHTMEYALQLAQTGHLCIFTIHANNAVQTIERIMNFYPEERHQQVLMDLALNTVGII
GQRLARLKGGGRTAIVDLLINSPAMQDHIFKGELMEAMSLMNRSETDGMQTFDQDLFRLYTEDKITYEEALRQAESVNDL
RLKIKLWEEGNAKDHIFERVGGLSLQ

Nucleotide

• Length: 1281 bp

>NTDB_id=561785 LVJ79_RS08635 WP_003787551.1 1621455..1622735(-) (pilU) [Kingella kingae strain 4177/66]
ATGGCAGAACAAGACATCACTTCCCTACTTGAAGGTTTTGTAAACAATTACAAAGTAACCAACGATGCCGCCAAGCCTGC
TGCACCAGCAGGACGAGCCGTGCCTGCATCGGCACAAGCCACGGCAACGGCTTCGGAAATTGCGCCAGCCAAAAAATCGG
GCTTACACCCTTTGTTGGAAAAAATGGCTGCCGAATGTGAAAAACGCGGTGCATCGGATATTTTTATCAGCGCAGGTTTT
CCGCCATCATTCAAAATCAATGGCGAATTAACGCCTGTTCCGCTCAAACCGCTCACGCAGCAAGACACGCAAATTTTGGT
GTATTCCACTATGTCGCCAGACCAACGTGCGCGTTTTGACCACGAATTGGAATTGAACTACTCCGTGTCGTCTAGCGAGG
GCATTCGCTTTCGTGTGAACTCGTATCACGAACAAGGTCGCATGGGCATGGTGTGGCGTCGCATCACCACCAAAATCTTA
ACGGTTGATGATTTGCAGTTGCCACAAGGTTTAAAAGACTTGGCGATGCAGCCGCGCGGTTTGTTGATTTTGGCAGGCGC
AACGGGTTCGGGTAAATCTACGTCTATGGCGGCGATGCTGGACTGGCGTAACAAACACGCCGCAGGTCATATTGTGACCA
TTGAAGACCCTGTGGAATATCTGCACAAACCAATCAAGAGTATTTTCACGCAACGCGAATTGGGCGTGGATACGAATTCT
TGGTCAATGGCGGTGCAATCGGCAATGCGTCAAGCACCAGACGTGGTTTGTGTGGGTGAGGTGCGTAACGAACACACCAT
GGAATACGCTTTGCAACTGGCGCAAACAGGTCACTTGTGTATCTTTACCATTCATGCGAACAATGCCGTGCAAACCATTG
AGCGTATTATGAACTTCTATCCAGAAGAGCGTCATCAACAAGTATTGATGGACTTGGCGTTGAACACAGTGGGCATTATT
GGTCAGCGTTTGGCGCGATTGAAAGGCGGCGGACGTACGGCGATTGTGGATTTGTTGATTAACAGCCCTGCGATGCAAGA
CCACATTTTCAAAGGCGAGTTGATGGAAGCGATGTCGTTGATGAATCGTTCGGAAACAGACGGCATGCAAACGTTTGACC
AAGACTTGTTCCGCTTGTACACCGAAGACAAAATCACTTACGAAGAAGCCTTGCGCCAAGCTGAATCGGTAAACGACTTG
CGCTTGAAAATCAAATTGTGGGAAGAGGGTAATGCCAAAGACCACATCTTTGAGCGCGTGGGCGGTTTGAGCTTGCAATA
A

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (96-326)

3D structure

• AlphaFold DB: F5S877

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	47.696	86.62	0.413
pilU	Acinetobacter baylyi ADP1	49.711	81.221	0.404
pilU	Vibrio cholerae strain A1552	46.429	78.873	0.366