Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KFK26_RS07100 Genome accession   NZ_CP073820
Coordinates   1494194..1495927 (+) Length   577 a.a.
NCBI ID   WP_011408072.1    Uniprot ID   A0A0K0GIN1
Organism   Xanthomonas oryzae strain PXO99A-gx     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1483564..1503088 1494194..1495927 within 0


Gene organization within MGE regions


Location: 1483564..1503088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFK26_RS07050 (KFK26_07050) - 1483657..1484151 (+) 495 WP_324250706.1 DUF6714 family protein -
  KFK26_RS07055 (KFK26_07055) - 1484218..1485537 (-) 1320 WP_115877354.1 IS701-like element ISXo15 family transposase -
  KFK26_RS07060 (KFK26_07060) - 1485674..1486642 (+) 969 WP_229002716.1 IS5-like element ISXo1 family transposase -
  KFK26_RS07065 (KFK26_07065) coaE 1486768..1487376 (-) 609 WP_011258357.1 dephospho-CoA kinase -
  KFK26_RS07070 (KFK26_07070) - 1487390..1488253 (-) 864 WP_011258358.1 A24 family peptidase -
  KFK26_RS07075 (KFK26_07075) pilC 1488260..1489519 (-) 1260 WP_011408067.1 type II secretion system F family protein Machinery gene
  KFK26_RS07080 (KFK26_07080) pilA2 1489874..1490299 (+) 426 WP_011408068.1 pilin Machinery gene
  KFK26_RS07085 (KFK26_07085) - 1490434..1492023 (+) 1590 WP_011408069.1 phosphoethanolamine transferase -
  KFK26_RS07090 (KFK26_07090) - 1492034..1492663 (+) 630 WP_011408070.1 hypothetical protein -
  KFK26_RS07095 (KFK26_07095) - 1492633..1494159 (+) 1527 WP_041182621.1 membrane protein -
  KFK26_RS07100 (KFK26_07100) pilB 1494194..1495927 (+) 1734 WP_011408072.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KFK26_RS07105 (KFK26_07105) - 1495936..1496196 (-) 261 WP_027703280.1 cell filamentation protein Fic -
  KFK26_RS07110 (KFK26_07110) - 1496284..1496445 (-) 162 WP_153296778.1 hypothetical protein -
  KFK26_RS07115 (KFK26_07115) - 1496507..1496713 (-) 207 WP_027703281.1 YhfG family protein -
  KFK26_RS07120 (KFK26_07120) pilR 1496786..1498237 (-) 1452 WP_011258365.1 sigma-54 dependent transcriptional regulator Regulator
  KFK26_RS07125 (KFK26_07125) - 1498508..1500121 (-) 1614 WP_012444444.1 HAMP domain-containing sensor histidine kinase -
  KFK26_RS07130 (KFK26_07130) sucC 1500355..1501524 (+) 1170 WP_011258367.1 ADP-forming succinate--CoA ligase subunit beta -
  KFK26_RS07135 (KFK26_07135) sucD 1501549..1502424 (+) 876 WP_012444445.1 succinate--CoA ligase subunit alpha -
  KFK26_RS07140 (KFK26_07140) - 1502597..1503088 (+) 492 WP_027703282.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62970.00 Da        Isoelectric Point: 5.4513

>NTDB_id=560984 KFK26_RS07100 WP_011408072.1 1494194..1495927(+) (pilB) [Xanthomonas oryzae strain PXO99A-gx]
MNSVSTTNLVGITGIARRLVQDGALEETAARTAMEQAAAAKVPLPQWFAENRLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=560984 KFK26_RS07100 WP_011408072.1 1494194..1495927(+) (pilB) [Xanthomonas oryzae strain PXO99A-gx]
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AACCGCTGCGCGGACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAACAGGCTGG
TGTCGGCGTCGCAGCTTGCGGCGGCCAATGCAGTGGAATTTGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTAGATGACATCAAGTTTCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATCCGTCGCACATTGGAGCAGTGGCAGGCAAGCAATGCCTCATTTGGATCATCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCT
GTACACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTTGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K0GIN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.042

98.44

0.562
  pilB Acinetobacter baumannii D1279779

56.69

98.44

0.558
  pilB Legionella pneumophila strain ERS1305867

52.465

98.44

0.516
  pilB Vibrio cholerae strain A1552

49.74

100

0.497
  pilF Neisseria gonorrhoeae MS11

49.648

98.44

0.489
  pilB Vibrio campbellii strain DS40M4

46.046

98.614

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

46.359

97.574

0.452
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

91.508

0.36