Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   KSO_RS07230 Genome accession   NC_020272
Coordinates   1412450..1413064 (+) Length   204 a.a.
NCBI ID   WP_015387983.1    Uniprot ID   A0A6A8LHQ3
Organism   Bacillus amyloliquefaciens IT-45     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1407450..1418064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSO_RS07195 (KSO_007405) aroE 1408137..1408979 (+) 843 WP_003152856.1 shikimate dehydrogenase -
  KSO_RS07200 (KSO_007410) yhbY 1408973..1409263 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  KSO_RS07205 (KSO_007415) - 1409274..1409843 (+) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  KSO_RS07210 (KSO_007420) yqeK 1409833..1410414 (+) 582 WP_015387982.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  KSO_RS07215 (KSO_007425) rsfS 1410411..1410767 (+) 357 WP_014305451.1 ribosome silencing factor -
  KSO_RS07220 (KSO_007430) - 1410764..1411501 (+) 738 WP_014305450.1 class I SAM-dependent DNA methyltransferase -
  KSO_RS07225 (KSO_007435) comER 1411570..1412391 (-) 822 WP_003152866.1 late competence protein ComER -
  KSO_RS07230 (KSO_007440) comEA 1412450..1413064 (+) 615 WP_015387983.1 helix-hairpin-helix domain-containing protein Machinery gene
  KSO_RS07235 (KSO_007445) - 1413131..1413700 (+) 570 WP_003152868.1 ComE operon protein 2 -
  KSO_RS07240 (KSO_007450) comEC 1413701..1416052 (+) 2352 WP_015387984.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  KSO_RS19515 - 1416071..1416206 (-) 136 Protein_1448 YqzM family protein -
  KSO_RS20030 - 1416247..1416401 (+) 155 Protein_1449 hypothetical protein -
  KSO_RS07245 (KSO_007455) holA 1416441..1417482 (+) 1042 Protein_1450 DNA polymerase III subunit delta -
  KSO_RS07250 (KSO_007460) rpsT 1417499..1417765 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22255.41 Da        Isoelectric Point: 5.5211

>NTDB_id=56093 KSO_RS07230 WP_015387983.1 1412450..1413064(+) (comEA) [Bacillus amyloliquefaciens IT-45]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=56093 KSO_RS07230 WP_015387983.1 1412450..1413064(+) (comEA) [Bacillus amyloliquefaciens IT-45]
ATGACGAAAGCAATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment