NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	KEC47_RS11915	Genome accession	NZ_CP073656
Coordinates	2518871..2519485 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus velezensis strain Y816
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2513871..2524485

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KEC47_RS11885 (KEC47_11885)	rpsT	2514171..2514437 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
KEC47_RS11890 (KEC47_11890)	holA	2514454..2515495 (-)	1042	Protein_2312	DNA polymerase III subunit delta	-
KEC47_RS11895 (KEC47_11895)	-	2515535..2515689 (-)	155	Protein_2313	hypothetical protein	-
KEC47_RS11900 (KEC47_11900)	-	2515730..2515864 (+)	135	WP_003152870.1	YqzM family protein	-
KEC47_RS11905 (KEC47_11905)	comEC	2515883..2518234 (-)	2352	WP_077722611.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
KEC47_RS11910 (KEC47_11910)	-	2518235..2518804 (-)	570	WP_003152868.1	ComE operon protein 2	-
KEC47_RS11915 (KEC47_11915)	comEA	2518871..2519485 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
KEC47_RS11920 (KEC47_11920)	comER	2519544..2520365 (+)	822	WP_014305449.1	late competence protein ComER	-
KEC47_RS11925 (KEC47_11925)	-	2520434..2521171 (-)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
KEC47_RS11930 (KEC47_11930)	rsfS	2521168..2521524 (-)	357	WP_003152864.1	ribosome silencing factor	-
KEC47_RS11935 (KEC47_11935)	yqeK	2521542..2522102 (-)	561	WP_077722612.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
KEC47_RS11940 (KEC47_11940)	-	2522092..2522661 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
KEC47_RS11945 (KEC47_11945)	yhbY	2522672..2522962 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
KEC47_RS11950 (KEC47_11950)	aroE	2522956..2523798 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=559745 KEC47_RS11915 WP_013352942.1 2518871..2519485(-) (comEA) [Bacillus velezensis strain Y816]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=559745 KEC47_RS11915 WP_013352942.1 2518871..2519485(-) (comEA) [Bacillus velezensis strain Y816]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0559745