NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	KEC47_RS11490	Genome accession	NZ_CP073656
Coordinates	2442362..2443432 (-)	Length	356 a.a.
NCBI ID	WP_070082113.1	Uniprot ID	-
Organism	Bacillus velezensis strain Y816
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2437362..2448432

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KEC47_RS11440 (KEC47_11440)	-	2437422..2438006 (-)	585	WP_012117977.1	signal peptidase I	-
KEC47_RS11445 (KEC47_11445)	tapA	2437978..2438649 (-)	672	WP_070082109.1	amyloid fiber anchoring/assembly protein TapA	-
KEC47_RS11450 (KEC47_11450)	-	2438908..2439237 (+)	330	WP_003153097.1	DUF3889 domain-containing protein	-
KEC47_RS11455 (KEC47_11455)	-	2439277..2439456 (-)	180	WP_003153093.1	YqzE family protein	-
KEC47_RS11460 (KEC47_11460)	comGG	2439513..2439890 (-)	378	WP_077722592.1	competence type IV pilus minor pilin ComGG	Machinery gene
KEC47_RS11465 (KEC47_11465)	comGF	2439891..2440286 (-)	396	WP_077722593.1	competence type IV pilus minor pilin ComGF	-
KEC47_RS11470 (KEC47_11470)	comGE	2440300..2440614 (-)	315	WP_077722594.1	competence type IV pilus minor pilin ComGE	Machinery gene
KEC47_RS11475 (KEC47_11475)	comGD	2440598..2441035 (-)	438	WP_077722595.1	competence type IV pilus minor pilin ComGD	Machinery gene
KEC47_RS11480 (KEC47_11480)	comGC	2441025..2441333 (-)	309	WP_003153087.1	competence type IV pilus major pilin ComGC	Machinery gene
KEC47_RS11485 (KEC47_11485)	comGB	2441338..2442375 (-)	1038	WP_063174751.1	competence type IV pilus assembly protein ComGB	Machinery gene
KEC47_RS11490 (KEC47_11490)	comGA	2442362..2443432 (-)	1071	WP_070082113.1	competence type IV pilus ATPase ComGA	Machinery gene
KEC47_RS11495 (KEC47_11495)	-	2443624..2444574 (-)	951	WP_003153082.1	magnesium transporter CorA family protein	-
KEC47_RS11500 (KEC47_11500)	-	2444720..2446021 (+)	1302	WP_077722596.1	hemolysin family protein	-
KEC47_RS11505 (KEC47_11505)	-	2446059..2446889 (-)	831	WP_070082115.1	STAS domain-containing protein	-
KEC47_RS11515 (KEC47_11515)	-	2447095..2447475 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
KEC47_RS11520 (KEC47_11520)	-	2447712..2447957 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40557.91 Da Isoelectric Point: 9.5862

                                >NTDB_id=559741 KEC47_RS11490 WP_070082113.1 2442362..2443432(-) (comGA) [Bacillus velezensis strain Y816]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFREAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=559741 KEC47_RS11490 WP_070082113.1 2442362..2443432(-) (comGA) [Bacillus velezensis strain Y816]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACCCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAC

ACAAGGGATGCTAAGGGCGCCATATACAGACTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATCGCTGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCGGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCGGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	81.742	100	0.817
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0559741