NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	KEF49_RS11825	Genome accession	NZ_CP073635
Coordinates	2362860..2363930 (-)	Length	356 a.a.
NCBI ID	WP_115997636.1	Uniprot ID	-
Organism	Bacillus amyloliquefaciens strain Ba13
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2357860..2368930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KEF49_RS11775 (KEF49_11775)	-	2357919..2358503 (-)	585	WP_212098460.1	signal peptidase I	-
KEF49_RS11780 (KEF49_11780)	tapA	2358475..2359146 (-)	672	WP_212098462.1	amyloid fiber anchoring/assembly protein TapA	-
KEF49_RS11785 (KEF49_11785)	-	2359404..2359733 (+)	330	WP_045510605.1	DUF3889 domain-containing protein	-
KEF49_RS11790 (KEF49_11790)	-	2359774..2359953 (-)	180	WP_016938971.1	YqzE family protein	-
KEF49_RS11795 (KEF49_11795)	comGG	2360010..2360387 (-)	378	WP_212098464.1	competence type IV pilus minor pilin ComGG	Machinery gene
KEF49_RS11800 (KEF49_11800)	comGF	2360389..2360889 (-)	501	WP_249199234.1	competence type IV pilus minor pilin ComGF	-
KEF49_RS11805 (KEF49_11805)	comGE	2360798..2361112 (-)	315	WP_065981881.1	competence type IV pilus minor pilin ComGE	Machinery gene
KEF49_RS11810 (KEF49_11810)	comGD	2361096..2361533 (-)	438	WP_045510590.1	competence type IV pilus minor pilin ComGD	Machinery gene
KEF49_RS11815 (KEF49_11815)	comGC	2361523..2361831 (-)	309	WP_115997634.1	competence type IV pilus major pilin ComGC	Machinery gene
KEF49_RS11820 (KEF49_11820)	comGB	2361836..2362873 (-)	1038	WP_212098466.1	competence type IV pilus assembly protein ComGB	Machinery gene
KEF49_RS11825 (KEF49_11825)	comGA	2362860..2363930 (-)	1071	WP_115997636.1	competence type IV pilus ATPase ComGA	Machinery gene
KEF49_RS11830 (KEF49_11830)	-	2364123..2365073 (-)	951	WP_212098468.1	magnesium transporter CorA family protein	-
KEF49_RS11835 (KEF49_11835)	-	2365220..2366521 (+)	1302	WP_212098471.1	hemolysin family protein	-
KEF49_RS11840 (KEF49_11840)	-	2366559..2367389 (-)	831	WP_212098474.1	STAS domain-containing protein	-
KEF49_RS11850 (KEF49_11850)	-	2367591..2367971 (-)	381	WP_115997640.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
KEF49_RS11855 (KEF49_11855)	-	2368207..2368452 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40486.69 Da Isoelectric Point: 8.8524

                                >NTDB_id=559599 KEF49_RS11825 WP_115997636.1 2362860..2363930(-) (comGA) [Bacillus amyloliquefaciens strain Ba13]

MENIETYSRNIINEAYMARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSSGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCETDNCSVYCRLYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=559599 KEF49_RS11825 WP_115997636.1 2362860..2363930(-) (comGA) [Bacillus amyloliquefaciens strain Ba13]

ATGGAGAATATTGAAACATACAGCAGGAACATCATCAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTGCC

GAGGGAAAGGGATGCTTTCATCCATTTTCGCATCGGTCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTTAAATTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGATCACTGTCT

CTGCCGCTTCCAACGGAAACCGTTCATTTGAGGATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

ATTGCCTAAGAGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCATTATTATCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTTTTTACCGGCCCGACCGGATCTGGAAAAACAACTACCCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTGGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCTCCGGTTTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG

GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGTGCGGCCATGACGGGCCACCTCGTACTTTCAAGCCTGCAT

ACAAGAGATGCCAAGGGCGCCATATACAGGCTGCTGGAGTTCGGGGTGAACATGACGGAAATTGAACAAACCGTAATTGC

CATCGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCGAAACGGACAACTGTTCCGTGTACTGCCGTCTGTACA

GGCAGACACGCCGTGCGGGGATTTATGAACTGCTTTACGGAAAAAATCTCACCCAGTGCTTTCAGGAAGCAAAAGGAGAG

CACGCCAATTTTCAATATCAGACGCTTCGCAGGCTGATCAGAAAAGGTATCGCGCTCGGTTATGTCACGACTGATAATTA

TGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.584	100	0.826
pilB	Glaesserella parasuis strain SC1401	38.506	97.753	0.376
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0559599