Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KC397_RS13875 Genome accession   NZ_CP073273
Coordinates   2937031..2937621 (-) Length   196 a.a.
NCBI ID   WP_020429291.1    Uniprot ID   A0AAX2LK04
Organism   Vibrio fluvialis strain 19-VB00936     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2932031..2942621
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KC397_RS13855 (KC397_13855) aroB 2932949..2934034 (-) 1086 WP_020329448.1 3-dehydroquinate synthase -
  KC397_RS13860 (KC397_13860) aroK 2934062..2934580 (-) 519 WP_004728569.1 shikimate kinase AroK -
  KC397_RS13865 (KC397_13865) pilQ 2934768..2936504 (-) 1737 WP_233921186.1 type IV pilus secretin PilQ family protein Machinery gene
  KC397_RS13870 (KC397_13870) pilP 2936529..2937041 (-) 513 WP_020429290.1 pilus assembly protein PilP Machinery gene
  KC397_RS13875 (KC397_13875) pilO 2937031..2937621 (-) 591 WP_020429291.1 type 4a pilus biogenesis protein PilO Machinery gene
  KC397_RS13880 (KC397_13880) pilN 2937614..2938204 (-) 591 WP_212570868.1 PilN domain-containing protein Machinery gene
  KC397_RS13885 (KC397_13885) pilM 2938194..2939198 (-) 1005 WP_212570869.1 type IV pilus assembly protein PilM Machinery gene
  KC397_RS13890 (KC397_13890) - 2939341..2941821 (+) 2481 WP_212570870.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22722.10 Da        Isoelectric Point: 4.7023

>NTDB_id=558733 KC397_RS13875 WP_020429291.1 2937031..2937621(-) (pilO) [Vibrio fluvialis strain 19-VB00936]
MANLQELELDEIAEWPLLPQLAVLLLLVLALQGAGYWLYMLPKQDALEGLKQQEQTLKSTIRIKASKVATLPKLKTQLDE
LAERYDFLLRQLPVQKELASMLASVNELGLENTLTFTRIDWGEKQNQEFLYRLPLNIELTGNYHNIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=558733 KC397_RS13875 WP_020429291.1 2937031..2937621(-) (pilO) [Vibrio fluvialis strain 19-VB00936]
ATGGCTAACTTGCAGGAACTGGAACTGGATGAAATCGCTGAATGGCCTTTGTTGCCTCAGCTGGCCGTGTTGTTACTGCT
GGTTCTGGCACTGCAAGGTGCGGGCTATTGGTTATATATGTTGCCCAAGCAGGATGCGCTCGAAGGTTTAAAACAGCAGG
AACAAACGCTCAAATCGACGATACGTATCAAAGCCAGCAAAGTGGCCACGTTACCCAAACTCAAAACCCAACTGGATGAA
CTGGCAGAACGGTATGACTTCCTGCTGCGTCAACTGCCGGTGCAAAAAGAGCTTGCCAGTATGCTGGCGTCGGTTAACGA
GCTGGGGCTGGAAAATACGCTGACGTTCACGCGGATTGACTGGGGGGAGAAGCAGAATCAGGAATTCCTCTATCGTCTGC
CACTCAATATCGAACTGACCGGCAATTATCACAACATCGGCGACTTTTCCGAAGCGATTGCGCGCTTGCCACGCATCATC
AACTTTGACGATGTCGATTGGCAGCGCGTCAGTCAGGAGAGCAGCACGCTGCACTTTCGCGTCCGCGCTTACACCTATCA
GTTCAAACCGGAGGTGAGCGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

79.592

100

0.796
  pilO Vibrio campbellii strain DS40M4

68.947

96.939

0.668