Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   KAJ74_RS13675 Genome accession   NZ_CP073012
Coordinates   2786365..2787339 (+) Length   324 a.a.
NCBI ID   WP_031587486.1    Uniprot ID   -
Organism   Staphylococcus aureus strain SA14+     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2781365..2792339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAJ74_RS13640 (KAJ74_13640) rpmG 2781825..2781974 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  KAJ74_RS13645 (KAJ74_13645) - 2782183..2782722 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  KAJ74_RS13650 (KAJ74_13650) - 2782734..2784197 (+) 1464 WP_001017858.1 rhomboid family intramembrane serine protease -
  KAJ74_RS13655 (KAJ74_13655) - 2784178..2784381 (+) 204 WP_000087561.1 YqgQ family protein -
  KAJ74_RS13660 (KAJ74_13660) - 2784378..2785364 (+) 987 WP_000161314.1 ROK family glucokinase -
  KAJ74_RS13665 (KAJ74_13665) - 2785364..2785693 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  KAJ74_RS13670 (KAJ74_13670) - 2785690..2786313 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  KAJ74_RS13675 (KAJ74_13675) comGA 2786365..2787339 (+) 975 WP_031587486.1 competence type IV pilus ATPase ComGA Machinery gene
  KAJ74_RS13680 (KAJ74_13680) comGB 2787311..2788381 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  KAJ74_RS13685 (KAJ74_13685) comGC 2788395..2788706 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  KAJ74_RS13690 (KAJ74_13690) comGD 2788684..2789130 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  KAJ74_RS13695 (KAJ74_13695) comGE 2789117..2789416 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  KAJ74_RS13700 (KAJ74_13700) comGF 2789334..2789831 (+) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  KAJ74_RS13705 (KAJ74_13705) - 2789928..2790074 (+) 147 WP_001792109.1 hypothetical protein -
  KAJ74_RS13710 (KAJ74_13710) - 2790064..2790588 (+) 525 WP_001015120.1 shikimate kinase -
  KAJ74_RS13715 (KAJ74_13715) gcvT 2790747..2791838 (+) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36955.99 Da        Isoelectric Point: 8.6529

>NTDB_id=557350 KAJ74_RS13675 WP_031587486.1 2786365..2787339(+) (comGA) [Staphylococcus aureus strain SA14+]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKADVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=557350 KAJ74_RS13675 WP_031587486.1 2786365..2787339(+) (comGA) [Staphylococcus aureus strain SA14+]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGATGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.691

100

0.997
  comGA Staphylococcus aureus N315

99.691

100

0.997