Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   J8G15_RS17180 Genome accession   NZ_CP072852
Coordinates   3697448..3699040 (+) Length   530 a.a.
NCBI ID   WP_210543695.1    Uniprot ID   -
Organism   Rhodoferax sp. PAMC 29310 strain PAMC29310     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3692448..3704040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8G15_RS17155 argS 3692675..3694372 (+) 1698 WP_210543691.1 arginine--tRNA ligase -
  J8G15_RS17160 - 3694394..3695002 (+) 609 WP_210543692.1 SPOR domain-containing protein -
  J8G15_RS17165 - 3695066..3695713 (+) 648 WP_210543693.1 thiol:disulfide interchange protein DsbA/DsbL -
  J8G15_RS17170 lptA 3695939..3696556 (+) 618 WP_210543694.1 lipopolysaccharide transport periplasmic protein LptA -
  J8G15_RS17175 lptB 3696553..3697350 (+) 798 WP_240538351.1 LPS export ABC transporter ATP-binding protein -
  J8G15_RS17180 rpoN/rpoN1 3697448..3699040 (+) 1593 WP_210543695.1 RNA polymerase factor sigma-54 Machinery gene
  J8G15_RS17185 - 3699139..3700380 (+) 1242 WP_210543696.1 class I SAM-dependent RNA methyltransferase -
  J8G15_RS17190 - 3700377..3700787 (+) 411 WP_210543697.1 putative toxin-antitoxin system toxin component, PIN family -
  J8G15_RS17195 - 3700768..3702396 (-) 1629 WP_210543698.1 FMN-binding glutamate synthase family protein -
  J8G15_RS17200 - 3702513..3703325 (+) 813 WP_210543699.1 YecA family protein -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58217.71 Da        Isoelectric Point: 4.7711

>NTDB_id=556420 J8G15_RS17180 WP_210543695.1 3697448..3699040(+) (rpoN/rpoN1) [Rhodoferax sp. PAMC 29310 strain PAMC29310]
MKPGLSLRVSQHLSLTPQLQQSIRLLQLSTLEMSQEVEQMLDENPFLEVSAEEPPREEFGLAQADAPVRNEDLDVEAGAH
APAEFTAEVRVSSETDAELSPLSADDGQSWDGDGSASMAPDDGEWGGEAKAHGNNLDGDSDADVTELARSQESLQSHLHR
QALALHLSEEDGAALRFLIESLNDDGYLEEALAELVDGLTGDDLEEREQMLHHFTVALSLLKTLEPVGVGARGLAECLTL
QLKALQSQDDIKLAQARTVAVALRICAQPMDVLARRDIKRLVQLCGVPDAAIREGIALIGHLEPKPGRRFADVERNIVVP
DVIVTQVGNGSQAQFRVRLNADVMPRLRVHDIYANALKQHRGGGTEASNYAALQQRLQEARWFIKNIQQRFDTILRVSNA
IVERQKSFFMHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTFELKFFFGSGLGTDSGGNASSTAVRALIKQ
FISAENLKKPLSDNQISEMLKEQGIDCARRTVAKYREGLRIAPASLRKDL

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=556420 J8G15_RS17180 WP_210543695.1 3697448..3699040(+) (rpoN/rpoN1) [Rhodoferax sp. PAMC 29310 strain PAMC29310]
ATGAAGCCTGGCCTGTCCCTTCGTGTTTCCCAGCACCTGTCGCTGACGCCGCAATTGCAGCAGTCCATCCGGCTGTTGCA
GCTGTCGACGCTGGAAATGAGCCAGGAAGTCGAGCAAATGCTGGACGAAAACCCGTTTTTGGAGGTGAGCGCAGAAGAGC
CGCCACGCGAAGAATTTGGACTGGCACAAGCCGATGCACCGGTCCGCAATGAGGATCTGGATGTGGAAGCCGGCGCGCAT
GCTCCTGCCGAGTTCACTGCGGAAGTCCGGGTGAGCAGCGAAACCGATGCCGAATTGTCGCCCCTGTCGGCAGACGACGG
GCAGAGCTGGGACGGCGACGGCAGTGCTTCCATGGCGCCCGATGATGGCGAGTGGGGGGGCGAGGCCAAGGCCCACGGCA
ACAACCTGGATGGTGACAGTGATGCCGATGTGACCGAACTGGCGCGCAGCCAGGAGTCGCTGCAAAGCCATTTGCACCGG
CAGGCCCTGGCTTTGCACCTGAGTGAAGAAGATGGCGCCGCCCTGCGTTTCCTGATTGAGTCGCTGAACGACGACGGTTA
CCTGGAAGAGGCGTTGGCTGAGTTGGTTGACGGCTTGACCGGCGATGACCTTGAAGAGCGGGAGCAAATGCTGCACCACT
TCACCGTTGCGCTGTCCTTGCTTAAAACATTGGAGCCTGTGGGAGTGGGTGCACGGGGTTTGGCCGAGTGTTTGACGCTT
CAACTGAAAGCTTTGCAGAGTCAAGACGACATCAAGTTGGCCCAGGCGAGAACGGTGGCGGTGGCCCTGCGCATCTGTGC
GCAGCCGATGGATGTTTTGGCGCGGCGTGACATCAAGCGTTTGGTGCAATTGTGCGGTGTGCCGGATGCGGCCATTCGTG
AGGGGATCGCACTGATTGGGCATCTGGAGCCCAAACCCGGACGCCGGTTTGCCGATGTAGAGCGCAATATCGTGGTGCCG
GACGTCATCGTCACTCAAGTGGGCAACGGATCGCAGGCGCAGTTCAGGGTTCGCCTCAACGCCGACGTGATGCCTCGCCT
GCGGGTGCATGACATCTATGCCAACGCACTCAAGCAACACCGGGGTGGCGGCACCGAGGCGTCCAACTATGCGGCGCTGC
AACAGCGTCTGCAGGAAGCGCGCTGGTTCATCAAAAATATCCAGCAGCGTTTCGACACGATTTTGCGGGTGAGCAACGCC
ATTGTGGAGCGCCAAAAGAGCTTTTTCATGCATGGCGAACTGGCCATGCGCCCGCTTGTGCTACGGGAAATTGCAGACGA
ACTGGGCTTGCACGAGTCCACCATCAGCCGTGTGACCACTGCCAAATACATGGCCACGCCATTCGGCACTTTTGAGTTGA
AATTCTTCTTCGGCTCCGGCTTGGGGACCGACAGTGGGGGCAATGCTTCCAGCACGGCAGTGCGGGCGCTGATCAAGCAG
TTCATCAGTGCCGAAAATCTGAAGAAGCCCCTCAGCGACAATCAGATCTCCGAGATGCTCAAAGAGCAGGGCATTGATTG
CGCTCGCCGCACCGTGGCCAAGTACCGGGAAGGGCTGCGAATTGCCCCCGCCTCGCTGCGAAAAGACCTGTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(145-353)

Sigma-54, DNA binding domain (Sigma54_DBD)

(373-528)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.991

100

0.504