Detailed information NTG0555481

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LL229_RS07810
• Genome accession: NZ_CP090823
• Coordinates: 1515142..1516464 (+)
• Length: 440 a.a.
• NCBI ID: WP_021214546.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain 229
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1510142..1521464

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL229_RS07785 (LL229_1225)	glyQ	1510658..1511611 (-)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-
LL229_RS07790 (LL229_1224)	-	1511896..1512642 (-)	747	WP_003130666.1	bifunctional metallophosphatase/5'-nucleotidase	-
LL229_RS07795 (LL229_1223)	-	1512758..1513777 (-)	1020	WP_003130663.1	DUF475 domain-containing protein	-
LL229_RS07800 (LL229_1222)	-	1513825..1514322 (-)	498	WP_003130662.1	VanZ family protein	-
LL229_RS07805 (LL229_1221)	-	1514456..1515085 (-)	630	WP_021214545.1	YigZ family protein	-
LL229_RS07810 (LL229_1220)	comFA	1515142..1516464 (+)	1323	WP_021214546.1	DEAD/DEAH box helicase	Machinery gene
LL229_RS07815 (LL229_03075)	comFC	1516707..1517111 (+)	405	WP_003130657.1	ComF family protein	Machinery gene
LL229_RS07820 (LL229_1218)	-	1517145..1517723 (-)	579	WP_003130655.1	nucleotidyltransferase family protein	-
LL229_RS07825 (LL229_1217)	-	1517737..1518192 (-)	456	WP_003130653.1	diacylglycerol kinase family protein	-
LL229_RS07830 (LL229_1216)	ybeY	1518176..1518664 (-)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
LL229_RS07835 (LL229_1215)	-	1518816..1519295 (-)	480	WP_003130650.1	NUDIX hydrolase	-
LL229_RS07840 (LL229_1214)	-	1519353..1520321 (-)	969	WP_003130649.1	PhoH family protein	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50187.69 Da
• Isoelectric Point: 10.1065

>NTDB_id=555481 LL229_RS07810 WP_021214546.1 1515142..1516464(+) (comFA) [Lactococcus lactis subsp. lactis strain 229]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDDFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=555481 LL229_RS07810 WP_021214546.1 1515142..1516464(+) (comFA) [Lactococcus lactis subsp. lactis strain 229]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGATTTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (114-233)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.25	90.909	0.675
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	38.018	98.636	0.375